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Characterizing the silica deposition vesicle of diatoms (1989)

Li, C. -W. ; Chu, S. ; Lee, M.

Springer

Protoplasma 151 (1989), S. 158-163

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ISSN: 1615-6102

Keywords: Lectins ; Mannose ; Silica deposition vesicles ; Ditylum brightwellii

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The present study on a centric diatom,Ditylum brightwellii, includes two parts: detection of sugars in the silica deposition vesicle (SDV) with lectins and labeling the developing siliceous cell wall in the SDV with rhodamine 123. Cells with developing valves are treated with SDS to remove all the cytoplasmic contents, then either stained with fluorescein labeled lectins or thin-sectioned and stained with colloidal gold labeled lectins. The results show that mannose is part of the organic matrix in the SDV. Rhodamine 123, a non-toxic fluorescent laser dye, enters the cell immediately and is trapped in the SDV probably by the high reducing potential of the SDV. Silica is co-deposited with rhodamine 123 in the SDV, and the resulting valves and girdle bands become fluorescent. Implications of this study for the mechanism of silicification are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01403453

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Evidence concerning the morphogenesis of saccular receptors in the bullfrog (Rana catesbeiana)Research sponsored by the Joint Services Electronics Program, Contract F44620-71-C-0087 and the United States Public Health Service, National Institute of Health, grant GM-17523-02. (1973)

Lewis, E. R. ; Li, C. W.

New York, NY : Wiley-Blackwell

Journal of Morphology 139 (1973), S. 351-361

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ISSN: 0362-2525

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: A dichotomy of hair-cell types has been found in the bullfrog sacculus, and considerable evidence supports the view that one type (“peripheral”) is transformed during macular growth to the other type (“central”). Between the periphery and the center of the macula, one finds a gradation of form from “peripheral” to “central” type. Occasionally in adults and more often in stage-26 tadpoles one finds the presumably younger peripheral type of hair cell occurring well beyond the limits of the macula proper. The apparent morphogenic sequence for saccular hair cells is (1) development of a kinocillum on an endolymphatic epithelial cell, (2) gradual transformation of microvilli into stereocilia, (3) growth of the stereocilia and development of kinociliary bulb, (4) achievement of final size and form.

Additional Material: 15 Ill.