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Changes in serum C-reactive protein levels in dogs with various disorders and surgical traumas (1993)

Yamamoto, S. ; Shida, T. ; Miyaji, S. ; [et al.]

Springer

Veterinary research communications 17 (1993), S. 85-93

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ISSN: 1573-7446

Keywords: acute phase proteins ; dogs ; C-reactive protein ; enzyme-linked immunoabsorbent assay ; inflammation ; serum ; surgery

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract The serum levels of C-reactive protein (CRP) produced as an inflammatory response in dogs with various disorders and surgical traumas were measured by enzyme-linked immunoabsorbent assay and slide reversed passive latex agglutination test (RPLA). The CRP levels were greatly increased 1–2 days after surgery in most of the dogs (n=29) subjected to surgery. These levels had markedly decreased by the time the sutures were removed. In dogs with various disorders (n=58), the serum CRP levels at first diagnosis were high in infectious diseases. In dogs from which paired serum samples were examined, the serum CRP usually showed a decrease with improvement in the condition (n=11) or a terminal increase (n=4) but, conversely, some showed an increase with improvement in the condition (n=3).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01839236

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Induction of the acrosome reaction in guinea pig spermatozoa by calmodulin antagonist W-7 (1986)

Nagae, T. ; Srivastava, P. N.

New York, NY : Wiley-Blackwell

Gamete Research 14 (1986), S. 197-208

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ISSN: 0148-7280

Keywords: calmodulin antagonist ; spermatozoa ; capacitation ; acrosome reaction ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: The effect of the calmodulin antagonist W-7 on the capacitation and the acrosome reaction of guinea pig spermatozoa was examined. The characteristic features of the acrosome reaction induced by W-7 were the dependence on the composition and pH of the medium and on the presence of sodium bicarbonate. The most effective concentration of W-7 for inducing the acrosome reaction was approximately 5 μM, which is far less than the Kd for calmodulin. Moreover, W-7 enhanced the ability of spermatozoa to acquire capacitation in a Ca2+-free medium. The spermatozoa induced to undergo the acrosome reaction by W-7 were capable of penetrating the zona-free hamster eggs. W-5, which has a lower affinity for calmodulin than W-7, also induced the acrosome reaction in the same manner as W-7. These results suggest that the naphthalenesulfonamide derivatives W-7 and W-5 can induce the acrosome reaction in guinea pig spermatozoa via capacitation in a pH-dependent, Ca2+-calmodulin-independent manner.

Additional Material: 8 Tab.