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  • 1
    Electronic Resource
    Electronic Resource
    Renal regeneration following D-serine induced acute tubular necrosisSupported by the Chicago and Illinois Heart Association. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 383-387 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Regeneration of the rat kidney was observed for six days after inducing acute tubular necrosis of the proximal pars recta with d-serine (80 mg/100g body weight). Regenerating cells appear by two days post-treatment, and re-epithelialization of the nephron is completed within six days, with the most mature cells approaching normal morphology. Regeneration originates from viable cells adjacent to the necrotic zone which divide and follow a template provided by the intact basement membrane. Transient, cytoplasmic regenerative activity among developing tubular cells is characterized by the presence of large, irregularly shaped nuclei, prominent nucleoli, abundant ribosomes and lysosomes, and abnormal mitochondrial configurations. Microfilaments appear to be involved in the formation of apical microvilli and the basal labyrinth of plasmalemmal convolutions. These data suggest that d-serine. inducedacute tubular necrosis of the proximal pars recta may be followed by rapid, patterned regeneration along an intact basement membrane, and that microfilaments are involved in differentiation of cellular morphology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091930305
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  • 2
    Electronic Resource
    Electronic Resource
    The fine structure of the thrombocyte in the dogfish (Mustelus canis) with special reference to microtubule orientationThis work performed in part at the Marine Biological Laboratory, Woods Hole, Massachusetts.The investigation was supported in part by grants AI 03767, HE 10002-01 and HE 08172, National Institutes of Health, and by Boston University Graduate School. (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 156 (1966), S. 203-214 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The cytoplasm of the dogfish (Mustelus canis) thrombocyte was found to contain microtubules. The tubules which measured 230 Å in diameter appeared singly and in groups. They were found in all parts of the cytoplasm but usually close to the perinuclear area. Similar arrangements of tubules were observed in eosinophils and hemoblasts, but not in the mature, smaller lymphocytes. The orientations of the perinuclear bundles frequently reflected the shape of the nuclei, appearing in furrows, indentations, and between lobes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091560208
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  • 3
    Electronic Resource
    Electronic Resource
    The origin of the phagocytic mononuclear cells of the peripheral bloodA second report of Studies on the Mononuclear Cells of the Blood. (1919)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 25 (1919), S. 26-53 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1000250104
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  • 4
    Electronic Resource
    Electronic Resource
    Rapid fixation and embedding method for immunocytochemical studies of tomato spotted wilt tospovirus (TSWV) in plant and insect tissues (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 24 (1993), S. 514-520 
    Details
    ISSN: 1059-910X
    Keywords: Microwave energy ; Immunolabelling ; Antigenicity ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A new rapid fixation and embedding technique using microwave energy was evaluated for immunolabelling and examination of ultrastructure of plant and insect cells. Tissues in gluteraldehyde-paraformaldehyde were fixed for fifteen seconds in a microwave at 100% power, and dehydrated. Microwave energy was then used to polymerize the London Resin White (LR White) acrylic resin during the embedding process. Embedded specimens were then thin sectioned (90 nm) and treated with anti-tomato spotted wilt tospovirus (TSWV) antiserum followed by protein A-gold label, or antisera against a TSWV encoded nonstructural protein followed by goat anti-rabbit gold label. Using this technique, structural and nonstructural proteins of TSWV were readily detected and specifically labelled in cells of the insect vector, the western flower thrips, Frankliniella occidentalis (Pergande), and in infected cells of the plant species, Emilia sonchifolia L. © 1993 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070240608
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  • 5
    Electronic Resource
    Electronic Resource
    Transient expression of the cytochrome P450 aromatase gene in elongating porcine blastocysts is correlated with uterine insulin-like growth factor levels during peri-implantation development (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 37 (1994), S. 1-11 
    Details
    ISSN: 1040-452X
    Keywords: IGF-I ; IGF-II ; Uterus ; Embryo ; Estrogens ; Aromatase P450 ; Pregnancy ; RIA ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The insulin-like growth factors (IGFs-I and -II) are mediators of cellular growth and differentiation. The expression of these growth factor genes is temporally and hormonally regulated in the uterus during pregnancy, suggesting potentially important roles in embryonic development, implantation, and successful progression of pregnancy. A known regulator of uterine IGF-I secretion is estrogen, which is produced by pre-implantation mammalian embryos of several species and whose amounts may be influenced by growth factors via their effects on the transcriptional activities of steroidogenic enzyme genes. We have previously proposed that within the uterine microenvironment, a positive feedback loop may link uterine secretion of IGFs with embryonic production of estrogens to maintain and coordinate the timing of biological signals essential for embryo development. The present study examined the temporal relationships between the levels of conceptus cytochrome P450 aromatase mRNA and protein and concentrations of IGF-I and -II in uterine luminal fluids of pigs. A DNA fragment encoding a highly conserved region among mammalian aromatase P450 proteins was isolated by hybridization screening of a porcine genomic DNA library with a human aromatase P450 cDNA fragment as probe. A synthetic oligopeptide DDVIDGYPVKKGTNI within this highly conserved region was used to generate an antiserum in sheep that recognized a protein of Mr 49,000 in Western blot analysis of porcine ovarian, placental, endometrial, and conceptus extracts. A radioimmunoassay (RIA) for aromatase P450 was established and validated using this antiserum. RIA demonstrated highest levels of aromatase P450 protein in extracts of days 10, 11, and 12 porcine conceptuses with significantly diminished levels in elongated conceptuses at days 15 and 18. In the conceptus, aromatase P450 was localized to the inner cell layer (hypoblast) of the trophectoderm. A major mRNA transcript of aproximately 3 kb in length was demonstrated by Northern blot analysis of conceptus RNA with a porcine aromatase P450 antisense RNA probe. The relative levels of aromatase P450 mRNA were higher in conceptuses at day 12 than at days 15 and 18, in parallel with the levels of aromatase P450 protein. RIA of uterine luminal fluids demonstrated maximal concentrations of IGF-I at day 12, which were significantly decreased by day 15, and increased concentrations of IGF-II by day 12, which were maintained until day 18 of pregnancy. These results demonstrate that the transient expression of conceptus aromatase P450 mRNA and protein in elongating pig blastocysts is coincident with their capacity to secrete estrogens and with the rapidly changing concentrations of IGFs withing the uterine microenvironment. These results suggest that regulation of aromatase P450 gene expression by IGFs may represent one mechanism by which uterine factors modulate an embryonic function (e.g., estrogen production) that elicits coordinate changes in the endometrium in preparation for implantation. © 1994 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080370102
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  • 6
    Electronic Resource
    Electronic Resource
    A contribution to the anatomy and development of the venous system of chelonia (1905)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 4 (1905), S. 453-485 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1000040406
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  • 7
    Electronic Resource
    Electronic Resource
    Lectin binding to gp60 decreases specific albumin binding and transport in pulmonary artery endothelial monolayers (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 149 (1991), S. 575-584 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effect of albumin binding to cultured bovine pulmonary artery endothelial cell (BPAEC) monolayers on the transendothelial flux of 125I-labelled bovine serum albumin (BSA) was examined to determine its possible role on albumin transcy-tosis. The transport of 125I-BSA tracer across BPAEC grown on gelatin- and fibronectin-coated filters (0.8 μm pore diam.) was affected by the presence of unlabelled BSA in the medium in that transendothelial 125I-BSA permeability decreased, reaching a 40% reduction at BSA concentrations equal to or greater than 5 mg/ml. BSA binding to BPAEC monolayers was saturated at concentration of 10 mg/ml with an apparent binding affinity of 6 x 10-7 M. In contrast, gelatin added to the medium altered neither 125I-BSA binding nor transport. Several lectins were tested for their ability to inhibit 125I-BSA binding and transport. One lectin, Ricinus communis (RCA), reduced 125I-BSA binding by 70% and transport by 40%. Other lectins, Ulex europaeus, Triticum vulgare, and Glycine max decreased neither 125I-BSA binding nor transport. The reduction of 125I-BSA transport by RCA was not observed in the presence of saturating levels of BSA, indicating that RCA influenced only the albumin-dependent component of transport. RCA, but not other lectins, precipitated a 60 kDa plasmalemmal glycoprotein from cell lysates of surface radioiodinated BPAEC monolayers. This 60 kDa glycoprotein appears to be the equivalent of gp60 identified previously as an albumin binding glycoprotein in rat microvascular endothelium. In summary, approximately 40% of albumin transport across' BPAEC monolayers is dependent on albumin binding. This component of albumin transport is inhibited by 80% by the binding of RCA to gp60. These results suggest that binding of albumin to gp60 on pulmonary artery endothelial cell membrane is a critical determinant of transendothelial albumin flux involving mechanisms such as plasmalemmal vesicular transcytosis.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041490329
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  • 8
    Electronic Resource
    Electronic Resource
    Role of albumin arginyl sites in albumin-induced reduction of endothelial hydraulic conductivity (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 141 (1989), S. 558-564 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We determined the effect of albumin on endothelial hydraulic conductivity (Lp) and the contributions of the positively charged arginyl and lysinyl residues of albumin in mediaing the effect. Studies were made using monolayers of cultured sheep pulmonary artery endothelial cells grown to confluence on polycarbonate filters. Water flux was measured as transendothelial hydrostatic pressure was varied from 5 to 20 cm H2 O. Lp was calculated from the slope of the relationship of water flux versus pressure. The Lp of endothelial monolayers perfused with albumin-free Hanks Balanced Salt Solution (HBSS) was compared to perfusion with HBSS containing either native albumin, or albumin in which the arginyl residues were modified by a condensation reaction with 1,2-cyclohexanedione (CHD-albumin), or albumin in which the lysinyl residues were modified by a substitution reaction with succinic anhydride (SC-albumin). Baseline Lp at 2.5 mg/ml native albumin was 1.6±0.1 × 10-6 cm/s/cm H2O compared to the filter Lp after removing cells of 3.0 ± 0.3 × 10-4 cm/s/cm H2O. Endothelial Lp increased by 60% when albumin concentration was decreased from 2.5 mg/ml to 0.5 mg/ml (P 〈 0.05), but did not change with an increase in concentration to 10 mg/ml. Albumin-free buffer and CHD-albumin increased endothelial Lp by 2.2 ± 0.3-fold and 1.9 ± 0.3-fold, respectively (P〈0.05). All endothelial Lp values were restored to baseline when the native albumin concentration was returned to 2.5 mg/ml. Excess l-arginine (2 × 10-3 M) inhibited the effect of native albumin and increased endothelial Lp 1.5 ± 0.02-fold (P〈0.05), but excess l-lysine (4 × 10-3) in the presence of native albumin had no effect on Lp. None of the perfusates altered the filter Lp value. Neutral dextran (70 kD), in contrast to native albumin, had no effect on endothelial Lp. These results indicate that albumin reduces the hydraulic conductivity of endothelial monolayers in a concentration-dependent fashion and that the arginyl residues of albumin are required for the response. The effect of albumin may be meditated by a charge interaction of albumin with the endo-thelium.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041410314
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  • 9
    Electronic Resource
    Electronic Resource
    Ultrastructure of developing muscle cells in the chick embryoThis investigation was supported by USPHS Training Grant STI GM-281 and USPHS Research Grant AM-04806. (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 116 (1965), S. 115-147 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Sectioned as well as negatively stained preparations of developing myotomal cells from the chick embryo were studied with the electron microscope. The application of these two techniques to similar material made it possible to observe developing muscle cells in successive stages of differentiation and at the same time visualize some of the isolated components making up these cells.The thick and thin filaments observed in the embryonic muscle were morphologically indistinguishable from those observed in adult muscle. The thin filaments, found randomly dispersed in the cytoplasm of the early cells, were present in large concentrations before the first appearance of thick filaments. The first observed thick filaments had lengths of 1.5 μ, equal to that of adult thick filaments. The appearance of large polyribosomes was correlated with the first appearance of the thick filaments. Single and small groups of ribosomes were always present. Early formed thick and thin filament aggregates were found in the same hexagonal array that is typical to the organization of myofilaments in adult myofibrils. Non-striated myofibrils, associated with a periodic transverse tubular system, were found before striated myofibrils could be observed. Some preliminary observations were made concerning the appearance of glycogen particles.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001160107
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  • 10
    Electronic Resource
    Electronic Resource
    Successful maintenance of suckling rat ileum in organ culture (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 155 (1979), S. 375-389 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Ileum from rats 4, 9, 11, 12, and 15 days old can best be maintained for 24 hours in a system using Hanks' Balanced Salt Solution without fetal calf serum, at 25°C and 21% O2. Suckling rat duodenum and jejunum were difficult to maintain well for 24 hours in this system or a variety of other systems that were tried. A temperature of 37°C hastened deterioration of duodenum, jejunum or ileum. With ileum, 3H-thymidine and 14C-leucine were increasingly incorporated into DNA and protein over the 24-hour period. Light microscopy, as well as scanning and transmission electron microscopy, showed very good preservation of the ileum after 24 hours. The addition to the medium of hydrocortisone, 1 μm, and thyroxine, 0.01 μm, alone or in combination, did not change DNA or protein synthesis, or morphology, possibly because of the relatively short (24 hour) time period. Our organ culture system emphasizes the differences between suckling rat ileum and the rest of the intestine, and provides a new tool for evaluating, over a 24-hour period, the developing rat small intestine.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001550307
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