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  • Cell & Developmental Biology  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Otoconial morphology of the developing chick (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 204 (1982), S. 83-87 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: We examined with scanning electron microscopy the otolithic organs of chick embryos aged 6 to 21 days. Otoconial forms not previously reported in adult birds or mammals were found. On the basis of the size and prevalence of various types of otoconia at different embryonic stages we organized the otoconial forms into a possible growth sequence starting with an early “double-fluted” or skeletal form and terminating with the normal mature form found in adults.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092040111
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Studies of otoconia in the developing chick by polarized light microscopy (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 174 (1985), S. 131-144 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Using polarized light microscopy we were able to observe the mineralization patterns of embryonic and neonatal chick otoconia. We compared preparations of freshly dissected material spread under mineral oil to material that had been treated with various fixatives and dehydration agents. We found that the standard fixation agent, glutaraldehyde, and some immersion oils etche embryonic chick otoconia but that fixation with 70% acetone or 70% alcohol followed by dehydration to 100% acetone or 100% alcohol left the otoconia intact. The size and shape of freshly dissected chick otoconia observed with polarized light microscopy were similar to those of acetone-fixed, critical-point-dried material examined by SEM. Embryonic forms of otoconia were found to have a fluted pattern that was different in morphology from otoconia found in hatched chicks and adults. Embryonic chick otoconia did not exhibit the multifaceted surface morphology seen in embryonic rat otoconia. Comparisons of the same fields of otoconia under phase contrast microscopy and polarized light microscopy indicated that the freshly dissected otoconia of embryos exhibit little or no unmineralized (nonbirefringent) material but that glutaraldehyde-fixed otoconia exhibited unmineralized areas where etching had occurred. Size frequency distributions of freshly dissected embryonic and mature otoconia in five ages of embryos and hatched chicks were consistent with a hypothesized developmental sequence of otoconia.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001740204
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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