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  • 1
    Electronic Resource
    Electronic Resource
    Light and electron microscopy of the new born sagittal suture (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 212 (1985), S. 81-89 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Morphogenesis and maturation of the sagittal suture in newborn C57B1/6J strain mice were studied using light and electron microscopy. Morphodifferentiation of the murine parietal bones progresses radially with the interposed sagittal suture, assuming a greater level of maturity at birth at a midpoint along its length. The presumptive suture develops in a sulcus, deeper posteriorly, more shallow anteriorly. Cells at the osteogenic front (OF) are distinguished from the surrounding fibrocytic cells by a number of distinctive characteristics: 1. increased cytoplasmic density; 2. extensive endoplasmic reticulum; 3. dispersed nuclear chromatin aggregates; 4. extensive surface projections; 5. close approximation.Mineralization of the developing parietal bone occurs extracellularly with the initial deposits of apatite crystals exhibiting no oriented relationship to either cellular or extracellular fibrillar elements.The majority of collagen fibers lie superior and inferior to the presumptive suture, oriented anteroposteriorly with their long axes parallel to the ectocranial surface. Other fiber bundles more intimately associated with the developing suture display a more random orientation.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092120112
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  • 2
    Electronic Resource
    Electronic Resource
    TGF-β3-Mediated tissue interaction during embryonic heart development (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 32 (1992), S. 152-159 
    Details
    ISSN: 1040-452X
    Keywords: Growth factor ; Morphogenesis ; Antisense RNA ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A critical process during early heart development is the formation of mesenchymal cells which will contribute to valves and septa of the mature heart. These cells arise by an epithelial-mesenchymal transformation of endothelial cells in the atrioventricular (AV) canal and outflow tract areas of the heart. Adjacent endothelial cells in the atrium and ventricle remain epithelial. A three-dimensional collagen gel culture system has been exploited to examine the interactions that mediate this transformation. The AV canal myocardium produces a stimulus that is transmitted through an intervening extra-cellular matrix to the AV canal endothelium. This interaction is regionally specific, such that ventricular myocardium does not provide an adequate stimulus and ventricular endothelium does not respond to the AV canal myocardial stimulus. Exogenous TGF-β1 (or TGF-β2) can complement ventricular myocardium to produce transformation by AV canal endothelium. A blocking antibody, effective against several TGF-β, prevents cell transformation. To identify the specific member of the TGF-β family that functions in situ, antisense oligonucleotides for each of the numbered TGF-β were topically added to AV canal explant cultures. Only the oligonucleotide targeted to TGF-β3 was an effective inhibitor of mesenchymal cell formation. Studies have been undertaken to localize specific mRNas by in situ hybridization and RNase protection assays. These assays have concentrated on the regional and temporal appearance of TGF-β2 and 3. Surprisingly, RNase protection assays with a TGF-β3 sense probe showed the presence of a transcript complementary to TGF-β3. Further analysis of this tissue interaction included the testing of a variety of signal transduction mechanisms including kinases, G-proteins, and intracellular calcium. Tissue interaction in the heart is a complex interaction in which regulation of the induction process occurs in both the inducing and target tissues. © 1992 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080320211
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of transforming growth factor β in the embryonic avian lens coincides with the presence of mitochondria (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Developmental Dynamics 203 (1995), S. 317-323 
    Details
    ISSN: 1058-8388
    Keywords: TGFβ ; Chicken lens ; Lens differentiation ; Mitochondria ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: During their maturation, lens cells lose all membrane bound organelles, including mitochondira. In chicken embryos this process begins in the central lens fibers beginning around embryonic day 12 (E12). Transforming growth factor β (TGFβ) is a multipotent growth modulator thought to play a role in numerous developmental processes. TGFβ1 has been localized to mitochondria in rat liver cells and muscle cells. In the present study, we examined the expression of TGFβ isoform mRNAs and proteins during chicken embryonic lens development. PCR analysis demonstrated TGFβ2 and TGFβ3 trasncripts in the lens epithelium and fibers throughout pre- and post-hatching development. TGFβ isoforms were detected throughout the lens epithelium and fibers early in development (E6). However by E19, the distribution of TGFβ2 and TGFβ3 transcripts and proteins coincided with regions of the lens that contained mitochondria. In addition, intense TGFβ staining was observed in the basal portions of the equatorial epithelial cells, a region with abundant mitochondria. Transcripts for TGFβ1 and TGFβ4 were not detected in any tissue or time frame examined. Similarly, no immunostaining for TGFβ1 was observed. ©1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1002030304
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  • 4
    Electronic Resource
    Electronic Resource
    Selective expression of the chicken platelet-derived growth factor α (PDGFα) receptor during limb bud development (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Developmental Dynamics 198 (1993), S. 14-21 
    Details
    ISSN: 1058-8388
    Keywords: PDGF ; Receptor ; Limb development ; Chicken ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Platelet-derived growth factor (PDGF) affects proliferation and differentiation of chicken limb bud mesoderm in vitro. However, no PDGF receptor has been characterized in the chicken wing bud in vivo. In this study, we used reverse transcription PCR (rtPCR), Northern blot analysis, and Western blot analysis to identify a molecule, in the developing wing bud, which represents the chicken homolog of the PDGFα receptor. The chicken PDGFα receptor mRNA was present in both mesoderm and ectoderm and all stages of the developing limb bud examined. Cultured limb bud mesoderm also expressed the PDGFα receptor transcript. In addition, the PDGFα receptor protein was present in whole limb buds and cultured limb bud mesoderm. Expression of the PDGFα receptor in cultured mesoderm was independent of the presence of ectoderm cells. The relative sizes of both the mRNA and protein for the PDGFα receptor in the chicken limb bud were similar to mammalian counterparts. Using similar approaches, neither the mRNA nor protein representing the chicken homolog of the PDGFβ receptor was detected. These data demonstrate for the first time that a PDGFα receptor is present in the embryonic chicken limb bud and may help regulate growth and differentiation of the embryonic limb. © 1993 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001980103
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    Paper (German National Licenses)
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