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Electrofusion for the pronuclear transplantation of mouse eggs (1987)

Tsunoda, Y. ; Kato, Y. ; Shioda, Y.

New York, NY : Wiley-Blackwell

Gamete Research 17 (1987), S. 15-20

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ISSN: 0148-7280

Keywords: nuclear transplantation ; electrofusion ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: The present study was undertaken to determine the efficiency of HVJ treatment and electrofusion for pronuclear transplantation in the mouse. The output voltage and duration of the pulses were fixed to 200 μsec at 10 V or to 150 μsec at 15 V for electrofusion, because the maximum rates of blastomere fusion of 2-cell embryos and development of fused embryos in vitro were obtained under these conditions. Although the proportion of eggs with fused karyoplast (78%) and the fused eggs developed to morulae or blastocysts (67%) was significantly lower than those obtained after HVJ treatment (94% and 94%), the proportion of pregnant recipients and young obtained after treatment of fused eggs was not significantly different between these two procedures.It is advised that electrofusion can be used as a fusogenic procedure for pronuclear transplantation in the mouse in some cases where HVJ cannot be applied.

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/mrd.1120170103

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Kato, Y. ; Tsunoda, Y.

New York, NY [u.a.] : Wiley-Blackwell

Molecular Reproduction and Development 36 (1993), S. 276-278