Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Cell & Developmental Biology  (7)
Source
Material
Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Migration of bone marrow lymphocytes demonstrated by selective bone marrow labeling with thymidine-H3 (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 168 (1970), S. 139-159 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Thymidine-H3 was injected into the femoral and tibial marrow (labeled marrow) of guinea pigs while the hind limb circulation was arrested temporarily and non-radioactive thymidine was administered systemically. Blood and lymphoid tissue radioautographs were subsequently examined for the presence of marrow-derived labeled cells.Small lymphocytes in the labeled marrow showed a wave of labeling, maximal at two to three days. Concurrently, labeled small lymphocytes appeared in the blood and lymphoid tissues, mainly the spleen and mesenteric lymph node. Their numbers were greatest at four to five days, and declined rapidly thereafter. At first they appeared predominantly in the splenic red pulp and throughout the lymph node cortex, including the subcapsular sinus. By four to five days they were also concentrated in the splenic white pulp, including periarteriolar lymphoid sheaths, and in the lymph node medullary cords. They were detected within medullary sinuses, hilar lymphatics and thoracic duct lymph. Labeled monocytes and large lymphoid cells also appeared in the blood and lymphoid tissues, mainly in the spleen.It is concluded that bone marrow is a major source of circulating newly-formed small lymphocytes many of which migrate rapidly into the spleen and mesenteric lymph node.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091680202
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The The non-thymic origin of lymphocytes (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 165 (1969), S. 109-112 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091650114
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The migration of lymphocytes from bone marrow to popliteal lymph nodes demonstrated by selective bone marrow labeling with 3H-thymidine in vivo (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 175 (1973), S. 737-746 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Guinea pig popliteal lymph nodes were examined by DNA radioassay and radioautography following the selective labeling of tibial and femoral marrow cells by intramyeloid injections of 3H-thymidine. The DNA radioactivity of the node increased for the first four days and at four to seven days exceeded that seen after an intraperitoneal injection of the same total dose of 3H-thymidine, indicating an export of radioactivity from the labeled marrow to the node. Simultaneously, radioautographic sections of the node revealed labeled cells indicative of an origin from marrow precursors. Small lymphocytes constituted 60-90% of the labeled cells and reached maximal numbers at four to six days. Most of them were observed in the cortex, including the subcapsular sinus, primary follicles, mantle zones around germinal centers, and the lumen and walls of post-capillary venules. However, the highest labeling indices of small lymphocytes occurred in the medulla, including the medullary cords, medullary sinuses and efferent lymphatic vessels. Labeled large mononuclear cells, including large lymphoid cells, monocytes and large blast cells, were confined almost exclusively to the cortex. A small number of labeled plasma cells was observed in the medullary cords. It is concluded that newly-formed bone marrow lymphocytes migrate continuously into immunologically quiescent lymph nodes and become widely distributed throughout the cortex and medulla, while some enter the recirculating small lymphocyte pool.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091750408
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Quantitative studies of lymphocytes and other cell populations in the bone marrow of neonatally thymectomized C3H mice (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 184 (1976), S. 325-333 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The effects of neonatal thymectomy on the development of the lymphoid, erythroid and granulocytic cell populations in mouse bone marrow have been assessed by quantitative techniques. The numbers per unit volume of bone marrow of 17 cell types were determined in neonatally thymectomized and sham thymectomized C3H mice at two, four and eight weeks of age, and compared with those of normal C3H mice. After neonatal thymectomy the numbers of small lymphocytes, large and medium-sized lymphoid cells, and erythroid cells reached normal levels at two weeks but fell progressively to 18%, 22% and 42% of normal, respectively, by eight weeks. In sham thymectomized mice these cell populations did not differ significantly from normal. Immature and mature granulocytes were elevated in numbers two weeks after either neonatal thymectomy or sham thymectomy, suggesting a transient non-specific stimulation of granulocytopoiesis. During continuous infusion of 3H-thymidine for ten days in neonatally thymectomized mice aged four weeks and eight weeks many bone marrow small lymphocytes remained unlabeled. The results demonstrate that early postnatal development of bone marrow lymphoid and erythroid cells proceeds normally in the absence of the thymus, in accord with the concept of the bone marrow as a primary site of lymphocyte production and differentiation. In addition, some slowly-renewing small lymphocytes in bone marrow appear to be thymus-independent cells.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091840307
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Surface morphology of bone marrow lymphocytes. I. Scanning electron microscopy of small lymphocytes bone marrow and spleen (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 192 (1978), S. 423-433 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Cell separation techniques and scanning electron microscopy (SEM) were used to characterize the surface morphology of small lymphocytes in mouse bone marrow. Lymphocyte-rich fractions and unfractionated suspensions of bone marrow and spleen cells from 9-10-week-old C3H male mice were glutaraldehyde-fixed, syringed onto gelatin-coated silver membranes, dehydrated in ethanol, infiltrated with amyl acetate, critical point dried, coated with gold-palladium and examined by SEM. High proportions of cells were retained on the membranes. Purified spleen small lymphocytes showed unimodal distribution curves for cell diameter (mode, 3.4 μm) and for number of surface microvilli (mode, 55-60). Bone marrow small lymphocytes were identified initially in lymphocyte-rich marrow fractions and in erythroblast-depleted marrow from polycythemic mice as well as in normal whole marrow. The cells resembled spleen small lymphocytes in size distribution and they showed microvilli. However, the number of visible microvilli was lower on small lymphocytes in the bone marrow (mode, 35-40) than in the spleen. While in each small lymphocyte population the total number of microvilli was greater on larger cells than on smaller ones, the density of microvilli per unit area of cell surface tended to decrease with increasing cell size. The results establish that the small lymphocytes in mouse bone marrow, mainly locally-produced immature cells, have villous surfaces, but the number of microvilli per unit cell surface area is less than that on peripheral small lymphocytes, as seen in the spleen. Neither in the bone marrow nor in the spleen are subpopulations of small lymphocytes distinguishable solely by numbers of microvilli. The findings suggest that microvilli on bone marrow small lymphocytes may undergo further development during post-mitotic maturation, surface receptor expression and migration of the cells to peripheral lymphoid tissues.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091920308
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Introduction (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 170 (1984), S. 252-252 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001700303
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Genesis of B lymphocytes in the bone marrow: Extravascular and intravascular localization of surface IgM-bearing cells in mouse bone marrow detected by electron-microscope radioautography after in vivo perfusion of 125I anti-IgM antibody (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 170 (1984), S. 349-365 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The role of mammalian bone marrow in generating surface IgM (sIgM)-bearing B lymphocytes is reviewed. Precursor cells in the marrow give rise to large, rapidly dividing cells bearing free cytoplasmic μ chains (cμ). The progeny of the large cμ+ cells form a population of small, nondividing cμ+ cells that mature into small lymphocytes, progressively expressing sIgM and other B-cell surface membrane components. Newly formed sIgM+ cells soon migrate through the bloodstream to the spleen and other lymphoid tissues, where they may die after a short lifespan or be activated to produce antibody molecules. The large-scale lymphocytopoiesis in the bone marrow thus maintains a population of rapidly renewed virgin B lymphocytes in the peripheral lymphoid tissues. This process continuously creates and selects B cell clones with the wide range of antibody specificites necessary to mediate primary humoral immune responses through postnatal life.A technique for perfusing radiolabeled anti-IgM antibodies in young mice has now permitted sIgM+ cells to be detected radioautographically in histological preparations of bone marrow under the electron microscope. Small sIgM+ lymphocytes are situated either singly or in small groups throughout the extravascular hemopoietic compartment of the bone marrow, often near sinusoid walls adjacent to late erythroblasts and reticular cells. Some regional concentrations of sIgM+ cells are apparent. sIgM+ cells also appear in transit through the sinusoidal endothelium and are markedly concentrated in the lumen of some sinusoids. Intrasinusoidal sIgM+ small lymphocytes have high densities of sIgM and long microvilli, on which sIgM molecules are concentrated. These studies reveal the localization and cell associations of specifically identified sIgM+ small lymphocytes in the extravascular marrow compartment and suggest that these cells may also undergo a transient intravascular storage and maturation phase. Use of this in vivo immunolabeling technique to detect other cell-surface markers may further elucidate the microenvironmental basis of B lymphocyte genesis in the bone marrow.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001700310
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...