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  • 1995-1999  (5)
  • Anorectal malformation  (2)
  • Cell culture  (2)
  • 15,16-Dimethoxy-biladienes-ab  (1)
  • 1
    Digitale Medien
    Digitale Medien
    A novel 16,24-dehydrobiladiene-ab system: The reaction of xanthobilirubic acid methyl ester with bromine (1995)
    Springer
    Monatshefte für Chemie 126 (1995), S. 201-209 
    Details
    ISSN: 1434-4475
    Schlagwort(e): 16,24-Dehydrobiladiene-ab ; Biladiene-ab ; Xanthobilirubic acid methyl ester ; Mesobilirubin-XIIIα ; Mesobiliverdin-XIIIα ; 14-Formyl-tripyrrinone ; 15,16-Dimethoxy-biladienes-ab
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Der Umsatz von Xanthobilirubinsäuremethylester mit Brom in Methanol ergibt in 20-prozentiger Ausbeute ein rotes Pigment, für das die bislang nicht bekannte 16,24-Dehydrobiladien-ab-Konstitution abgeleitet wurde. Darüber hinaus konnte das entsprechende Mesobiliverdin-XIIIα, Mesobilirubin-XIIIα, 14-Formyltripyrrinon, und zwei diastereomere 15,16-Dimethoxybiladiene-ab aus der Reaktionsmischung isoliert werden. Die mechanistischen Aspekte dieser Reaktion werden diskutiert.
    Notizen: Summary On treating xanthobilirubic acid methyl ester with bromine in methanol, a red bile pigment, which was assigned the hitherto unknown 16,24-dehydrobiladiene-ab constitution, is obtained in 20% yield. In addition, the corresponding mesobiliverdin-XIIIα, mesobilirubin-XIIIα, 14-formyl-tripyrrinone, and two diastereomeric 15,16-dimethoxybiladienes-ab could be isolated from the reaction mixture. The mechanistic aspects of this reaction are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00812248
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  • 2
    Digitale Medien
    Digitale Medien
    The use of augmented-pressure colostography in imperforate anus (1997)
    Springer
    Pediatric surgery international 12 (1997), S. 383-385 
    Details
    ISSN: 1437-9813
    Schlagwort(e): Key words Colostogram ; Imperforate anus ; Anorectal malformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Colostomy is recommended in most newborns with imperforate anus (IA), except those with low-type anorectal malformations (ARM). Accurate demonstration of the anatomy of any associated fistula between the rectum and the urogenital tract is essential for optimal surgical management. Augmented-pressure distal colostography is recommended to fulfill this requirement prior to definitive surgical repair of IA. We examined 12 cases of high or intermediate-type ARM using this technique, which clearly demonstrated the distal perineal anatomy. All were confirmed at posterior sagittal anorectoplasty.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s003830050157
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  • 3
    Digitale Medien
    Digitale Medien
    The use of augmented-pressure colostography in imperforate anus (1997)
    Springer
    Pediatric surgery international 12 (1997), S. 383-385 
    Details
    ISSN: 1437-9813
    Schlagwort(e): Colostogram ; Imperforate anus ; Anorectal malformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Colostomy is recommended in most newborns with imperforate anus (IA), except those with low-type anorectal malformations (ARM). Accurate demonstration of the anatomy of any associated fistula between the rectum and the urogenital tract is essential for optimal surgical management. Augmented-pressure distal colostography is recommended to fulfill this requirement prior to definitive surgical repair of IA. We examined 12 cases of high or intermediate-type ARM using this technique, which clearly demonstrated the distal perineal anatomy. All were confirmed at posterior sagittal angorectoplasty.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01076945
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  • 4
    Digitale Medien
    Digitale Medien
    Establishment of cell lines derived from oyster, Crassostrea gigas Thunberg and hard clam, Meretrix lusoria Röding (1999)
    Springer
    Methods in cell science 21 (1999), S. 183-192 
    Details
    ISSN: 1573-0603
    Schlagwort(e): Cell culture ; Clam ; Mollusc ; Oyster
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The present study attempts to establish cell culture systems for the oyster, Crassostrea gigas Röding and the hard clam, Meretrix lusoria Thunberg. Treatment with collagenase was better than trypsin at dissociating mollusc tissue fragments for in vitro culture. Heart tissue of oyster and hard clam proved to be the most promising target tissue for the establishment of cell lines in vitro. Primary cultures of clam heart were established and successfully maintained for more than 5 months. Collagenase at a concentration of 100 μg/ml may enhance the growth of oyster and hard clam heart cell cultures in vitro.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009829807954
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  • 5
    Digitale Medien
    Digitale Medien
    Establishment of cell culture systems from penaeid shrimp and their susceptibility to white spot disease and yellow head viruses (1999)
    Springer
    Methods in cell science 21 (1999), S. 199-206 
    Details
    ISSN: 1573-0603
    Schlagwort(e): In vitro ; Cell culture ; Prawn tissues
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Monolayer cultures were established from ovary, heart, lymphoid tissue and peripheral hemocytes of penaeid shrimps including Penaeus monodon, P. japonicus and P. penicillatus. The most favorable conditions for the culture of penaeid shrimp cells in vitro was in CMRL and L-15 tissue culture media when used within an osmolarity range of 620--760 mmol/kg. The optimal maintenance temperature was 25 °C for tissues of P. japonicus and 28 °C for tissues of P. monodon and P. penicillatus. Among the four tissues tested, lymphoid tissue, or 'Oka organ', was superior to the other tissues for the formation of confluent cell monolayers. Cell cultures from lymphoid tissue and ovary have been subcultured up to three times. When peripheral hemocytes and heart were cultured, a maximum survival of 4 days was obtained. In contrast, cell cultures derived from ovary and lymphoid tissue were maintained alive for at least 20 days in appropriate culture systems. Neither confluent cell sheet nor adherence of cells was obtained in cultivation of hepatopancreas using the present culture systems. The results obtained from the present study also revealed that ovary extract, muscle extract and lobster hemolymph enhanced the survival of the cultured cells of penaeid shrimp in vitro. When the 'Oka organ' cell monolayer was incubated with either white spot disease virus (WSDV) or yellow head virus (YHV), no cytopathic effect (CPE) was obtained. However, at 5--7 days after establishment, significant CPE (a few foci) was observed in cell monolayers derived from WSDV- and YHV-infected Oka tissue. By electron microscopy, virions of WSDV and YHV were observed in the nuclei and cytoplasm of cultured cells. The CPE foci developed further with increased incubation time.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009885929335
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