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Digitale Medien

Culture and cryopreservation of adult human oligodendrocytes and astrocytes (1984)

Kim, S. U. ; Moretto, G. ; Ruff, B. ; [weitere]

Springer

Acta neuropathologica 64 (1984), S. 172-175

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Details

ISSN: 1432-0533

Schlagwort(e): Adult human ; Oligodendrocyte ; Astrocyte ; Tissue culture ; Cryopreservation ; Bulk isolation ; Immunofluorescence microscopy ; Cell type-specific markers

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Summary Human adult oligodendrocytes were bulkisolated from the brains obtained at autopsy by the Percoll density gradient method and cultured in vitro for 2–28 days. Cultured human oligodendrocytes were then scraped from the dishes or coverslips, frozen at −70°C in the presence of 10% dimethyl sulfoxide as a cryoprotectorant, and then stored in liquid nitrogen tank. After the fast thawing, 55%–70% of human oligodendrocytes plus astrocytes were found to survive in the culture. Identification of cell types was established by using antibodies directed against galactocerebroside (oligodendrocyte) and glial fibrillary acidic protein (astrocyte).

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00695582

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