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  • 1
    Electronic Resource
    Electronic Resource
    Different effect of methylazoxymethanol on mouse cerebellar development depending on the age of injection (1985)
    Springer
    Experimental brain research 57 (1985), S. 279-285 
    Details
    ISSN: 1432-1106
    Keywords: Cerebellum ; Development ; Methylazoxymethanol ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Methylazoxymethanol (MAM), a powerful antimitotic, has been extensively used to affect rodent CNS development. Here we show that MAM causes different effects on mouse cerebellum depending on the age of the injected pup. Sublethal doses were determined for each age. A single injection at birth permanently reduces the number of cells. In addition, the cytoarchitecture was greatly perturbed: Purkinje cells retained an immature aspect and were dispersed through the cerebellar cortex. A single dose of MAM injected into 5 day old mice also affected the number of cells but, at the level of light microscopy, the cytoarchitecture of the cerebellar cortex appeared not to be altered. Purkinje cells, however, showed some immaturity and degenerated around the 22nd postnatal day. This modulation of MAM effect appears to provide a good model for studying cerebellar ontogeny and neuronal plasticity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236533
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ectopic granule cell layer in mouse cerebellum after methyl-azoxy-methanol (MAM) treatment (1991)
    Springer
    Experimental brain research 86 (1991), S. 90-96 
    Details
    ISSN: 1432-1106
    Keywords: Development ; Immunohistochemistry ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Previous results from our laboratory (Bejar et al. 1985) indicated that a single injection in mouse pups of the antimitotic/mutagenic agent methylazoxymethanol at postnatal day 5 typically produces hypogranular cerebella with no changes in foliation, in contrast to the severe alterations observed after the more usual injection on the day of birth. Here we report that injection of a higher dose (30 mg/kg) of methylazoxymethanol, always at postnatal day 5, leads to the additional presence of a ectopic cell layer in adult cerebellum. Immunostaining with several antibodies recognizing cell specific proteins ruled out the possibility that these ectopic cells were glial and electron microscopy indicated that they were morphologically mature granule cells. In the molecular layer of other cerebellar areas and apparently unrelated with granule cell ectopia, ectopic Golgi epithelial cells were observed. The reason for the presence of these ectopic cells of different type in the molecular layer was discussed in relation with analogous ectopias obtained by other means.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231043
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Musciomol and flunitrazepam binding sites in a subcellular fraction enriched in rat cerebellar glomeruli (1992)
    Springer
    Neurochemical research 17 (1992), S. 683-686 
    Details
    ISSN: 1573-6903
    Keywords: Cerebellar glomeruli ; GABAA receptors ; muscimol ; flunitrazepam
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In the internal granular layer of the cerebellar cortex the polysynaptic complexes called glomeruli consist mainly of homogeneous populations of glutamatergic and GABAergic synapses, both located on granule cell dendrites. A subcellular fraction enriched in glomeruli was prepared from rat cerebellum, and the distribution of GABAA and of benzodiazepine binding sites between membranes derived from this fraction (fraction G) and from a total cerebellar homogenate (fraction T) was studied. The benzodiazepine and GABA binding sites were measured by the binding of agonists [3H]flunitrazepam and [3H]muscimol, respectively. The results indicate that both binding sites are present, but only slightly enriched, in the glomerular synapses. We found a muscimol/flunitrazepam binding site ratio of two, which is consistent with the enrichement of muscimol binding sites in the granular layer shown by both autoradiographic with radioactive glutamatergic ligands and in situ hybridization experiments respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00968005
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    Paper (German National Licenses)
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