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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Der Nervenarzt 67 (1996), S. 650-658 
    ISSN: 1433-0407
    Schlagwort(e): Schlüsselwörter Stereotaktische Biopsie ; Stereotaktische Operationen ; Zerebraler Abszeß ; Key words Stereotactic biopsy ; Stereotactic guided surgery ; Cerebral haematoma evacuation ; Cerebral abscess
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Summary CT- or MRI-guided stereotactic procedures should be a standard in a modern neurosurgical unit. Analysing 71 cases the indications and results of stereotactic neurosurgery are presented. In 53 patients stereotactic serial biopsies of different intraaxial lesions were performed, in 5 patients a spontaneous haemorrhage of the basal ganglia was removed by lysis with r-tPA. In 3 patients suffering from hydrocephalus due to diencephalic cysts a cystventricular shunt device was implanted. In 3 patients an intracerebral abscess was aspirated and drained. A stereotactic guided craniotomy and excision of small deep-seated lesions was performed in 6 cases. The accuracy of stereotactic tumour biopsies was 88.7 % in our series, in accordance to other authors. The stereotactic aspiration and drainage of an intracerebral abscess provides accurate localization and minimal cortical damage and offers the possibility of intracavitary application of antibiotics. The stereotactic internal shunt implantation seems to be an alternative approach in the treatment of diencephalic cysts due to its minimal invasiveness and low operative risk. The aspiration of basal ganglia haematomas with insertion of an external drainage allows the lysis of the haematoma with r-tPA or urokinase. With stereotactic guidance small, deep-seated intraaxial lesions can be well localized and removed.
    Notizen: Zusammenfassung CT- oder MRT-geführte stereotaktische Eingriffe sollten zum Standard eines modernen neurochirurgischen Zentrums gehören. Anhand der Analyse von 71 eigenen Fällen wird das Indikationsspektrum stereotaktischer Operationen dargestellt. Bei 53 Patienten wurden CT-geführte Serienbiopsien intrazerebraler Raumforderungen durchgeführt, bei 3 Patienten ein intrazerebraler Abszeß punktiert, aspiriert und drainiert, bei 5 Patienten Stammganglienblutung mit r-tPA lysiert, in 3 Fällen ein zystoventrikulärer Shunt bei dienzephalen Zysten implantiert. Bei 6 Patienten wurden stereotaktisch geführte Trepanationen zur Tumor- oder Angiomentfernung vorgenommen. Die stereotaktische Biopsie bietet alle Vorteile eines minimal-invasiven Verfahrens. Entsprechend den Mitteilungen anderer Autoren ergibt sich auch in unserer Serie eine Güte der Biopsie von 88,7 %. Die stereotaktische Punktion und Aspiration eines Hirnabszesses mit Insertion einer externen Drainage bietet neben dem Vorteil einer geringen Gewebetraumatisierung und exakten Lokalisation die Möglichkeit zur intrakavitären Antibiotikainstillation. Die stereotaktische Implantation eines zystoventrikulären Shunts stellt aufgrund des minimal-invasiven Charakters und der geringen Komplikationsrate ein alternatives Behandlungskonzept dar. Die stereotaktische Punktion der Hämatomkavität mit Insertion einer externen Drainage bietet neben der Aspiration flüssiger Hämatomanteile zur akuten Entlastung auch die Möglichkeit einer Lyse des Resthämatoms mit Urokinase oder r-tPA nach angiographischem Ausschluß einer Gefäßmalformation. Mithilfe der stereotaktisch geführten Kraniotomie können auch kleine, subkortikal gelegene Prozesse exakt lokalisiert und exstirpiert werden.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Pflügers Archiv 324 (1971), S. 1-25 
    ISSN: 1432-2013
    Schlagwort(e): Adenosine Triphosphatase ; Plasma Membrane Enriched Fraction ; Microsomes ; Kidney ; ATPase ; zellmembranangereicherte Fraktion ; Mikrosomen ; Niere
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A fraction enriched with plasma cell membranes (PMF) was isolated from rat kidney homogenate by differential centrifugation. Before NaJ treatment electron micrographs of the preparation showed a membraneous fraction with only a small contamination of mitochondria. After treatment with NaJ the residual PMF exhibited a low microsomal glucose 6-phosphatase activity. Marker enzymes of other subcellular fractions were not detected. The NaJ extracted PMF revealed a high specific activity of ATPase, 91% of which was stimulated by Na+ and K+. The MgNaK-ATPase was characterized by Michaelis Menten kinetics. In contrast, Hill coefficients (“n”) of NaK-ATPase for the activation by Na+, K+ and Mg-ATP were greater than one. Experiments with various nucleotide tri-, di- and monophosphates revealed a high substrate specificity of the NaK-ATPase. The pH optimum was in the range of 7.2. SH-reagents and ouabain depressed the Na+ and K+ stimulated enzyme activity. PMF isolated from rat kidneys exhibited an acylphosphatase and a nitrophenylphosphatase activity, both of which were stimulated by K+. Furthermore 5′-nucleotidase and leucine aminopeptidase activities were present in the fraction.—Thus, NaK-ATPase of the PMF revealed the typical properties of the NaK-ATPase demonstrated in the microsomal preparations, which has been referred to by Skou (1965) as the enzymatic basis of active cation transport.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Pflügers Archiv 324 (1971), S. 26-42 
    ISSN: 1432-2013
    Schlagwort(e): Renal Plasma Cell Membranes ; ATPase Activities ; Adrenalectomy ; Corticosteroids ; Niere ; zellmembranangereicherte Fraktion ; ATPase Aktivität ; Adrenalektomie ; Corticosteroide
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A plasma membrane enriched fraction (PMF) from rat kidney homogenates with a high specific activity of a Mg2+, Na+ and K+ activated ATPase was investigated. For comparison ATPase activity was measured also in the microsomal fraction. Adrenalectomy resulted in an increase of specific activities of Mg-, MgNaK- and NaK-ATPase in the PMF and a decrease of specific activities in the microsomal fraction. The increase of enzyme activities in the PMF has been demonstrated with three different techniques of ATPase preparation: with NaJ- or DOC-treatment and dialysis. The changes were more prominent when the adrenalectomized rats received tap water instead of saline as drinking fluid and thus were Na deprived. Kinetic studies revealed similarK m values for Na+, K+ and MgATP in control and adrenalectomized rats.V max values, however, increased about two-fold in the adrenalectomized animals. There was no difference in the allosteric activitation of the NaK-ATPase in both animal groups as indicated by equal “n” values. The increase in ATPase activity could be prevented by substitution with daldosterone plus dexamethasone although the plasma concentration of Na remained unaltered low because of Na+ deprivation. Cortisone exerted no influence on ATPase activity in adrenalectomized rats. From the data presented it is inferred that neither the activity of the ATPase enzyme system of the PMF nor that of the microsomal fraction is directly regulated by aldosterone. It is concluded that the changes observed are due to an adaptation to altered renal Na+ reabsorption which in turn is regulated by corticosteroids. This adaptive process is different in different subcellular fractions of rat tissue.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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