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  • 2000-2004  (2)
  • 1965-1969
  • Cell subculture  (1)
  • Chebyshev polynomials  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Annals of the Institute of Statistical Mathematics 52 (2000), S. 557-573 
    ISSN: 1572-9052
    Keywords: Chebyshev polynomials ; convex combination ; extremal problems for polynomials ; Lagrange interpolation polynomial ; optimal discrimination designs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract The extrapolation design problem for polynomial regression model on the design space [−1,1] is considered when the degree of the underlying polynomial model is with uncertainty. We investigate compound optimal extrapolation designs with two specific polynomial models, that is those with degrees |m, 2m}. We prove that to extrapolate at a point z, |z| 〉 1, the optimal convex combination of the two optimal extrapolation designs |ξ m * (z), ξ2m * (z)} for each model separately is a compound optimal extrapolation design to extrapolate at z. The results are applied to find the compound optimal discriminating designs for the two polynomial models with degree |m, 2m}, i.e., discriminating models by estimating the highest coefficient in each model. Finally, the relations between the compound optimal extrapolation design problem and certain nonlinear extremal problems for polynomials are worked out. It is shown that the solution of the compound optimal extrapolation design problem can be obtained by maximizing a (weighted) sum of two squared polynomials with degree m and 2m evaluated at the point z, |z| 〉 1, subject to the restriction that the sup-norm of the sum of squared polynomials is bounded.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-0603
    Keywords: Macrobrachium nipponense ; Cell subculture ; pH ; Zn2+
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cell culture system was devised for muscle cell of Macrobrachium nipponense in the study. The juvenile and adult shrimps were held in laboratory aquaria with penicillin 1000 IU/ml and streptomycin 1000 µg/ml for 12–24 hours. Cell cultures were established in medium 199 supplemented with 20% fetal bovine serum, 1 g/L glucose, 5.2 g/L NaCl, 1.43 g/L CaCl2, 0.05 g/L MgCl2, 100 IU/mL penicillin and 100 µg/ml streptomycin. Fibroblast-like cells were passaged up to three times and survived for 54 days. The results showed the optimum for subculture in vitro was in medium 199 with pH 7.6. Moreover, basal medium supplemented with Zn2+ 60 µg/L could enhance the growth of the muscle cells. It was found that better results for cell culture would be obtained more easily with juvenile shrimps caught in spring than adults in summer or autumn; and shrimps caught within 12 hours after ecdysis could grow much better than the intermoult shrimps.
    Type of Medium: Electronic Resource
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