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  • 1
    Electronic Resource
    Electronic Resource
    Photoaffinity labeling of the DDT1 MF-2 cell α1-adrenergic receptor (1985)
    Springer
    Molecular and cellular biochemistry 67 (1985), S. 47-53 
    Details
    ISSN: 1573-4919
    Keywords: α1-adrenergic receptor ; membrane ; photoaffinity ; smooth muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary In this study, we have used an α1-adrenergic receptor photoaffinity ligand, 2-[4-(4-azido-3-iodo-benzoyl)-piperazin-1-yl]-4-amino-6, 7-dimethoxyquinazoline (125I-APD), to label covalently the α1-adrenergic receptor in a smooth muscle cell line. Our results indicate that in the absence of light, (125I)APD binds reversibly to a site in the DDT1 MF-2 cell membranes having pharmacological characteristics of an α1-adrenergic receptor. Following incorporation of (125I)ADP into partially purified membranes a single labeled band of protein with a Mr of 81 000 was visualized by autoradiography following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Incorporation of (125I)-APD into this band was affected by adrenergic agonists and antagonists in a manner consistent with an α1-adrenergic interaction. Prazosin (α1-selective) blocked incorporation of the label into the Mr = 81 000 protein while yohimbine (α2-selective) did not. Of the adrenergic agonists, (−)-epinephrine and (−)-norepinephrine but not (−)-isoproterenol blocked labeling of the Mr − 81 000 protein. We conclude that the ligand binding site of the DDT1 MF-2 cell α1-adrenergic receptor resides in a Mr = 81 000 protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220985
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  • 2
    Electronic Resource
    Electronic Resource
    Glucocorticoid induction of β-adrenergic receptors in the DDT1 MF-2 smooth muscle cell line involves synthesis of new receptor (1987)
    Springer
    Molecular and cellular biochemistry 74 (1987), S. 21-27 
    Details
    ISSN: 1573-4919
    Keywords: glucocorticoid receptor ; β-adrenergic receptor regulation ; smooth muscle ; adenylate cyclase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We have shown that glucocorticoids induce the appearance of β2-adrenergic receptors in membranes of the ductus deferens smooth muscle cell line (DDT1 MF-2). A concomitant increase in isoproterenol stimulated adenylate cyclase activity in the absence of exogenously applied GTP was observed as was a significantly increased (p 〈 0.05) sensitivity of the adenylate cyclase system to exogenously applied GTP. However, no significant difference in the maximal velocity of adenylate cyclase between control and steroid treatment was measurable in the presence of sodium fluoride. Induction of β2-adrenergic receptors in DDT1 MF-2 cells is correlated with the presence of steroid receptors (androgen and glucocorticoid) in the cells since estrogens and progesterones had no effect on receptor levels. Finally, utilizing dense amino acid labeling of cells to measure old versus newly synthesized receptor sites by a density shift method, we have documented that glucocorticoid induction of β2-adrenergic receptors involves synthesis of new receptor protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221909
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of orientation and chemical structure on the strength properties of some polymers (1977)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 17 (1977), S. 229-233 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The stress-strain properties of oriented polymers are measured parallel and perpendicular to the direction of hot stretching. The polymers include styrene-acrylonitrile copolymers, vinyl alcohol copolymers, acrylic acid copolymers, polyelecf trolytes, and a high density polyethylene. Polarity and hydrogen bonding tend to increase the strength, especially in the direction perpendicular to the direction of hot stretching. Strong electrostatic bonding in polyelectrolytes and too much polar bonding in styrene-acrylonitrile & polymers decrease the strength by reducing the molecular mobility, which is required to prevent brittleness.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760170404
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  • 4
    Electronic Resource
    Electronic Resource
    Transitions in polymer melts (1977)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 17 (1977), S. 713-714 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: It is shown that the liquid-liquid transition Tll in polymer melts need not be a true polymer transition but can be an artifact of the torsional braid analysis technique. The shift in Tll with molecular weight then is due to the variation of viscosity with molecular weight.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760171002
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    Paper (German National Licenses)
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