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Sequence analysis, gene expression, and chromosomal assignment of mouse Borg4 gene and its human orthologue (2000)

Osada, N. ; Kusuda, J. ; Suzuki, Y. ; [et al.]

Springer

Journal of human genetics 45 (2000), S. 374-377

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ISSN: 1435-232X

Keywords: Key words Cell motility ; Binder of Rho GTPase ; CDC42 TC10 ; Borg4 ; Full-length cDNA ; Fluorescence in situ hybridization

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract The Borg (binder of Rho GTPases) family proteins interact with CDC42 and TC10 in a guanosine triphosphate (GTP)-dependent manner. We have isolated a full-length cDNA of the mouse Borg4 gene, which is a member of this family. Sequence analysis revealed that this gene encoded a putative 349-amino acid protein. By reverse transcription — coupled polymerase chain reaction (RT-PCR) analysis, we observed that Borg4 was expressed ubiquitously in adult tissues. Additionally, we determined the entire cDNA sequence of the putative human Borg4 orthologue. By fluorescence in situ hybridization, mouse Borg4 and the putative human orthologue have been assigned to mouse chromosome 11E and human chromosome 17q24–25, which has been described as syntenic to the mouse region.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s100380070012

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Pulse response in an immobilized-enzyme column: Theoretical method for predicting elution curves (1980)

Adachi, S. ; Hashimoto, K. ; Matsuno, R. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 22 (1980), S. 779-797

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: A theoretical method for predicting the elution profile of a pulse response in an immobilized-enzyme column is proposed. The method is based on a mass balance model, which is extensively used in gel chromatography. To test the method, a pulse of sucrose solution was applied to a column of spherical acrylamide gel in which was entrapped invertase from yeast, and it was eluted with 0.05M acetate buffer at pH 5.0. The elution curves of the substrate and the product were in fairly good agreement with the theoretically calculated ones. The method was extended to the system of reversible as well consecutive reactions.

Additional Material: 9 Ill.