ISSN:
0173-0835
Schlagwort(e):
Chemistry
;
Biochemistry and Biotechnology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
A method is presented for the incorporation of [35S]methionine into proteins from freshly microdissected brain tissue samples. Rats were killed by decapitation, the brain was rapidly removed and sectioned without freezing using a vibratome. Hypothalamic nuclei and circumventricular organs of interest were removed by microdissection and incubated in a media containing [35S]methionine for six hours. Proteins within these tissue samples were then separated by two-dimensional polyacrylamide gel electrophoresis. The gels were stained with silver, photographed, dried and exposed to autoradiographic film. The results suggest that 1) this technique will prove useful in examining the uptake of radiolabeled amino acids into proteins from discrete nuclei and other brain areas, and 2) there is considerable regional heterogeneity in the uptake of [35S]methionine by proteins from the different brain regions examined in this study.
Zusätzliches Material:
2 Tab.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/elps.1150050210
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