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  • Chemistry  (2)
  • 1,5-bisphosphate carboxylase  (1)
  • 1
    Digitale Medien
    Digitale Medien
    A point mutation in the N-terminus of ribulose-1,5-bisphosphate carboxylase affects ribulose-1,5-bisphosphate binding (1991)
    Springer
    Planta 184 (1991), S. 35-39 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Ribulose ; 1,5-bisphosphate carboxylase ; Gene mutation (N-terminus) ; Substrate affinity (ribulose-1,5-bisphosphate)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Mutagenesis in vitro of the gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/ oxygenase (EC 4.1.1.39) from Anacystis nidulans was used to generate novel enzymes. Two conserved residues, threonine 4 and lysine 11 in the N-terminus were changed. The substitution of threonine 4 with serine or valine had little effect on the kinetic parameters. The substitution of lysine 11 with leucine, which is non-polar, increased the K m for ribulose-1,5-bisphosphate from 82 to 190 μM but its replacement with glutamine, which has polar properties, had no appreciable effect.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00208233
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  • 2
    Digitale Medien
    Digitale Medien
    Further applications for capillary gas chromatography in routine quantitative toxicological analyses (1990)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 13 (1990), S. 754-758 
    Details
    ISSN: 0935-6304
    Schlagwort(e): Capillary GC ; Forensic ; Toxicology ; Drug analysis ; Biological fluids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: The use of capillary column gas chromatography for drug screening in forensic toxicology has become increasingly widespread. Screening procedures however are often lengthy and unsuitable for rapid confirmatory or quantitative applications. In order to develop a practical scheme for confirmatory/quantitative analysis, we have optimized a series of temperature profiles to allow the rapid quantitative determination of a wide range of acid/neutral and basic drugs in extracts from post mortem fluids and tissue. The appropriate profile is selected based on the retention index on a standard crosslinked methyl silicone column used to screen extracts. The use of a 5% phenyl methyl silicone phase allows complementary identification, and allows the separation of some pairs of compounds with identical retention indices in the screning procedure.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jhrc.1240131106
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  • 3
    Digitale Medien
    Digitale Medien
    Fast-Atom bombardment mass spectrometry of the organometallic parasiticide, meglumine antimonate (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 372-376 
    Details
    ISSN: 0951-4198
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Physik
    Notizen: The mass spectrometry of an organometallic parasiticide used for treatment of leishmaniasis was studied using primarily fast-atom bombardment with complementary studies conducted using electrospray ionization. The positive-ion mass spectrum of meglumine antimonate contained ions with characteristic antimony isotope ratios (57:43) at m/z 627/629, 507/509, 406/408 and 314/316. There was also evidence of ion clusters containing antimony atoms (Sb1-3), which decompose to the mass 507/509 species under acid pH conditions. For meglumine antimonate, an empirical formula C14H30O10N2Sb (molecular weight 507) is proposed with a structure containing four Sb—O bonds in a symmetrical geometry.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/rcm.1290090503
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