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  • 1
    ISSN: 1432-1106
    Keywords: Retinal ganglion cells ; Cell death ; Trophic factors ; Tissue culture ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The enzyme horseradish peroxidase (HRP) was injected into the visual centres of the brains of neonatal rats. Following dissociation of retinae into tissue culture, the ganglion cells could be identified by appropriate histochemical staining for HRP reaction product. Cultures were prepared of dissociated retinae from rats aged 2–6 days postnatal. After 3 h the cultures were fixed, and HRP-labelled cells visualized and counted. Estimates were made of the number of ganglion cells per retina at each age. Results indicated a loss of ganglion cells during the first few postnatal days. This loss paralleled that observed in vivo. It was further found the retinal ganglion cells died rapidly in vitro when cultured in a minimal medium. Only 50% of ganglion cells originally plated remained viable after 24 h. However, the survival rate could be increased to 100% by coculturing the cells with diencephalon and mesencephalon; these contain the retinorecipient nuclei. Coculturing with cerebellum did not result in such an enhanced survival rate. Ganglion cells could be maintained over longer periods of time by reinoculating the cultures with additional tissue containing diencephalon and mesencephalon. These results support the hypothesis that developing neurons require trophic factors from their target tissues in order to survive.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 327 (1985), S. 317-327 
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: EPR-Investigations on the Photolysis of Halogenated Organic Compounds. II. CHBr3 and CBr4 in Glassy and Polycrystalline MatricesPhotolysis of CHBr3, CDBr3 and CBr4 in glassy and polycrystalline matrices (alcohols, diethylether, benzene derivatives and KBr) at T ≥ 77 K yields stationary e.p.r.-spectra in the range of effective g-factor from 2.2 to 1.9. By comparison of the spectra of CHBr3 and CDBr3, exposed to γ-rays and u.v. light respectively, and the spectra of these compounds in matrices it was shown that the photolysis in matrices produces the species \documentclass{article}\pagestyle{empty}\begin{document}$ {\rm CBr}_4\,^{_{\bar.}} $\end{document}, ·CBr3 and α-Br-radicals from the halogenated compounds and HĊO, ·CH3, CH3ĊHOH, RĊHOH, and ·C2H5 from the matrix. Photolysis of halogenated compounds in CH3OH and CD3OD yields only HĊO and ·CH3 together with a very small amount of bromine-containing radicals. In all other investigated matrices the concentration of bromine containing species is controlled by physical and chemical properties of the matrix. Time development of the \documentclass{article}\pagestyle{empty}\begin{document}$ {\rm CBr}_4\,^{_{\bar.}} $\end{document}-radical and of those generated from the matrix is different and gives insight into the complex photoreaction in solid matrices.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 329 (1987), S. 569-578 
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Aryltropylium salts exhibit an unusually effective self quenching of fluorescence in dependence on their concentration in dichlòromethane. The quenching process is investigated by static and time resolved fluorescence measurements. The observed biexponential fluorescence decay is interpreted by the emissions of ion pairs and free cations. The rate constant of fluorescence quenching by formation ion pair is two orders of magnitude greater than the diffusion constant.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 324 (1982), S. 925-932 
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: About the Deactivation Behaviour of Photoexcited Aryltropyliumions. II. Fluorescence Quenching of AryltropyliumionsThe properties of aryltropyliumions in the excited singlet state were studied. These ions are very strong acceptors. So the fluorescence is quenched by aromatic donors. The rates of fluorescence quenching depend on the ionization potential of the donor. This fact is discussed in terms of mechanisms derived by WELLER et al. The results were related to the light stability of aryltropyliumions.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 327 (1985), S. 310-316 
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: EPR-Investigations on the Photolysis of Halogenated Organic Compounds I. Pure Halogenated Compounds (CBr4, CHBr3, CCl4 and CHCl3)EPR-spectra of CBr4, CHBr3 and CDBr3, exposed to u.v.-light and γ-rays respectively, are presented. They are discussed and compared with the results of the photolysis of CH2Br2, CH3Br and CCl4, CHCl2, CH2Cl2 and CH3Cl. Under stationary conditions at 77K it was possible to identify paramagnetic species like \documentclass{article}\pagestyle{empty}\begin{document}$ {\rm CBr}_4\,^{_{\bar.}} $\end{document}, ·CBr3, ·CH3, ·C2H5, ·CCl3 and trapped electrons.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 325 (1983), S. 104-112 
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Electrochemistry of Organic Cations. Arylsubstituted Tropylium SaltsThe electrochemical behaviour of some mono- and diarylsubstituted tropylium perchlorates in acetonitrile was investigated and is discussed in connection with their u. v.-vis absorption spectra.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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