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Digitale Medien

Cortical perineuronal nets in the gray short-tailed opossum (Monodelphis domestica): a distribution pattern contrasting with that shown in placental mammals (1998)

Brückner, G. ; Härtig, W. ; Seeger, J. ; [weitere]

Springer

Anatomy and embryology 197 (1998), S. 249-262

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ISSN: 1432-0568

Schlagwort(e): Key words Neocortex ; Hippocampus ; Extracellular space ; Perineuronal net ; Marsupials

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract Extracellular matrix proteoglycans accumulated in perineuronal nets and in certain neuropil zones have been shown to influence the immediate neuronal microenvironment, and to contribute to the chemoarchitectonic characteristics of neuronal networks. Studies in different placental mammals, including the human, have suggested that the major principles of extracellular matrix distribution remained constant during phylogenesis of the different mammalian strains. However, the comparison of matrix distribution between various species also indicates that striking deviations from the basic pattern may occur, although their functional significance appears unknown as yet. This study examines the extracellular matrix in the forebrain of a basic American marsupial, which has evolved independently of placental mammals for more than 100 million years. Brain sections obtained from adult gray short-tailed opossums (Monodelphis domestica) were stained for extracellular matrix components using the N-acetylgalactosamine-binding lectin Wisteria floribunda agglutinin (WFA), a polyclonal antibody against chondroitin sulfate proteoglycans (CSPG), and biotinylated hyaluronectin for the detection of hyaluronan. In subcortical regions, the distribution patterns of WFA-stained and CSPG-immunoreactive perineuronal nets were similar to those reported previously in placental mammals. In contrast, a unique distribution was found in the neocortex. This distribution was characterized by the presence of perineuronal nets around pyramidal cells and matrix components within the adjacent neuropil that together form a continuously labeled zone in layer V. Weakly stained nets ensheathed less numerous pyramidal cells in the upper layers II/III and a few multipolar cortical neurons. Dual staining experiments showed that cortical net-associated neurons were rarely immunopositive for parvalbumin. This fact, in addition to the predominant association of extracellular matrix components with layer V pyramidal cells, differentiates the neocortex in Monodelphis from that of all placental mammals studied to date. Regarding the basic phylogenetic position of this marsupial species it remains to be shown if these distribution characteristics of extracellular matrix may represent also a basic feature of cortical organization.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004290050135

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Digitale Medien

Synthesis of nanoparticles for brain cell labelling in vivo (1992)

Paulke, B.-R. ; Härtig, W. ; Brückner, G.

Weinheim : Wiley-Blackwell

Acta Polymerica 43 (1992), S. 288-291

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ISSN: 0323-7648

Schlagwort(e): Chemistry ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie , Physik

Beschreibung / Inhaltsverzeichnis: Durch Emulsionscopolymerisation wurden verschiedenartige Latices synthetisiert, die sich in ihrer Partikelgröße, der Hydrophilie des Polymers und der Oberflächenbedeckung mit funktionellen Gruppen unterschieden. Die Partikel wurden mittels Einschlußpolymerisation, kovalenter Bindung des Fluoreszenz-Farbstoffes während der Teilchenbildung und deswachstums bzw. nachträglicher Oberflächenmodifizierung der Latices mit intensiver Fluoreszenz ausgestattet. Konzentrierte Suspensionen der Teilchen wurden in das Hirngewebe der Maus injiziert. Die intrazelluläre Markierung von GliaZellen nach erfolgter Phagozytose und der retrograde Transport von Teilchen in Neuronen durch 2 unterschiedliche Typen fluoreszierender Latices konnten an Gewebeschnitten nachgewiesen werden.

Notizen: Different types of latices were synthesized by emulsion copolymerization that differ in particle size, polymer hydrophilicity and surface coverage with functional groups. All of these particles were equipped with intensive fluorescence by inclusion polymerization, covalent bonding during particle formation and growth or surface modification of pre-fabricated latices, respectively. Concentrated particle suspensions were injected into the brain tissue of mice. The filling of glial cells by phagocytosis and the retrograde labelling of neurons with two kinds of beads could be shown in brain sections.

Zusätzliches Material: 3 Ill.