ISSN:
0951-4198
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Physics
Notes:
Deuterium-exchange experiments were performed on ribonuclease A (RNase A) and its glycosylated form, ribonuclease B (RNase B), using electrospray ionization mass spectrometry. The number of exchangeable protons was found to be similar for both forms of the enzyme, indicating that the oligosaccharide moiety on RNase B does not exert any influence on protein conformation. Four main glycoform populations were identified in RNase B, each of which was homogeneous and associated with one phosphate group. Spectra for RNase A were heterogeneous due to the presence of 0-3 phosphate groups bound to the enzyme.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/rcm.1290070505
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