ISSN:
0006-3592
Schlagwort(e):
chemometrics
;
light scattering
;
microbial productivity
;
Chemistry
;
Biochemistry and Biotechnology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
This article describes the use of chemometric methods for prediction of biological parameters of cell suspensions on the basis of their light scattering profiles. Laser light is directed into a vial or flow cell containing media from the suspension. The intensity of the scattered light is recorded at 18 angles. Supervised learning methods are then used to calibrate a model relating the parameter of interest to the intensity values. Using such models opens up the possibility of estimating the biological properties of fermentor broths extremely rapidly (typically every 4 sec), and, using the flow cell, without user interaction. Our work has demonstrated the usefulness of this approach for estimation of yeast cell counts over a wide range of values (105-109 cells mL-1), although it was less successful in predicting cell viability in such suspensions. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:131-143, 1998.
Zusätzliches Material:
15 Ill.
Materialart:
Digitale Medien
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