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  • Chemistry  (4)
  • in situ hybridization  (4)
  • satellite DNA  (4)
  • 1
    Digitale Medien
    Digitale Medien
    High-resolution mapping of repetitive DNA by in situ hybridization: molecular and chromosomal features of prominent dispersed and discretely localized DNA families from the wild beet species Beta procumbens (1996)
    Springer
    Plant molecular biology 30 (1996), S. 1099-1113 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Beta procumbens ; Beta vulgaris ; in situ hybridization ; repetitive DNA ; satellite DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Members of three prominent DNA families of Beta procumbens have been isolated as Sau3A repeats. Two families consisting of repeats of about 158 bp and 312 bp are organized as satellite DNAs (Sau3A satellites I and II), whereas the third family with a repeat length of 202 bp is interspersed throughout the genome. Multi-colour fluorescence in situ hybridization was used for physical mapping of the DNA families, and has shown that these tandemly organized families occur in large heterochromatic and DAPI positive blocks. The Sau3A satellite I hybridized exclusively around or near the centromeres of 10, 11 or 12 chromosomes. The Sau3A satellite family I showed high intraspecific variability and high-resolution physical mapping was performed on pachytene chromosomes using differentially labelled repeats. The physical order of satellite subfamily arrays along a chromosome was visualized and provided evidence that large arrays of plant satellite repeats are not contiguous and consist of distinct subfamily domains. Re-hybridization of a heterologous rRNA probe to mitotic metaphase chromosomes revealed that the 18S-5.8S-25S rRNA genes are located at subterminal position on one chromosome pair missing repeat clusters of the Sau3A satellite family I. It is known that arrays of Sau3A satellite I repeats are tightly linked to a nematode (Heterodera schachtii) resistance gene and our results show that the gene might be located close to the centromere. Large arrays of the Sau3A satellite II were found in centromeric regions of 16 chromosomes and, in addition, a considerable interspersion of repeats over all chromosomes was observed. The family of interspersed 202 bp repeats is uniformly distributed over all chromosomes and largely excluded from the rRNA gene cluster but shows local amplification in some regions. Southern hybridization has shown that all three families are specific for genomes of the section Procumbentes of the genus Beta.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00019545
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  • 2
    Digitale Medien
    Digitale Medien
    Comparative analysis of the chromosomal and genomic organization of Ty1-copia-like retrotransposons in pteridophytes, gymnosperms and angiosperms (1997)
    Springer
    Plant molecular biology 33 (1997), S. 11-21 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Arabidopsis thaliana ; ferns ; in situ hybridization ; phylogenetics ; pines ; reverse transcriptase genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have investigated the physical distribution of the reverse transcriptase genes of Ty1-copia-like retrotransposable elements from 12 plant species belonging to different subdivisions by hybridization in situ on chromosome preparations. Ty1-copia-like elements showed different and non-random hybridization patterns. A dispersed distribution throughout most of the chromosomes with reduced hybridization at some regions or with some weak clustering at other regions was found in Allium cepa, Beta vulgaris, Brassica campestris, Brassica oleracea, Pennisetum glaucum, Pinus elliottii, Selaginella apoda, Vicia faba and Vicia narbonensis. Reduced hybridization occured mainly at centromeric regions, nucleolus-organizing regions and regions known to be mainly composed of tandemly repeated sequences. In the fern Pteris cretica the retroelements showed a dispersed genomic organization with clustering at some chromosomal regions and whole chromosomes showing little signal. In Arabidopsis thaliana and Cicer arietinum, Ty1-copia-like elements were found in clusters at the paracentromeric heterochromatin, a novel organization for a repetitive element in A. thaliana. New retroelement families were isolated from A. thaliana and from Beta vulgaris. Alignment of the deduced peptide sequences with Ty1-copia-like elements from other plants showed considerable divergence which was used to calculate their relationships, indicating the value of reverse transcriptase gene analysis in phylogenetic and biodiversity studies.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1005797222148
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  • 3
    Digitale Medien
    Digitale Medien
    Analysis of a repetitive DNA family from Arabidopsis arenosa and relationships between Arabidopsis species (1995)
    Springer
    Plant molecular biology 27 (1995), S. 853-862 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Arabidopsis arenosa ; Cruciferae ; genus Arabidopsis ; in situ hybridization ; molecular systematics ; satellite DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have analysed a family of highly repetitive DNA from Arabidopsis arenosa (L.) Lawalrée [syn. Cardaminopsis arenosa (L.) Hayck] composed of AT-rich tandem repeats of 166–179 bp in head to tail organization. Sequence comparison between several repeat units revealed a high level of divergence of 4.5% to 25%. The sequence family shows more than 58% homology to satellite sequences described in Arabidopsis thallana (L.) Heynh. but no homology to other satellite repeats in the Cruciferae. Within the genus Arabidopsis the satellite sequence was found to be present in A. thaliana and Arabidopsis suecica (Fries) Norrlin, but not in Arabidopsis griffithiana (Boiss.) N. Busch and Arabidopsis pumila (Stephan) N. Busch. In situ hybridization to metaphase chromosomes of A. arenosa (2n=4x=32) showed the sequence to be localized at the centromeres of all 32 chromosomes with substantial hybridization along the chromosome arms. Using Southern hybridization and in situ hybridization, we give evidence that A. suecica is a hybrid of A. thaliana and A. arenosa. A considerable reorganization of the A. thaliana satellite sequence pAL1 occurred in the hybrid genome while no molecular change of the A. arenosa repeat was observed in the hybrid. Analysis of related repeats enabled differentiation between closely related genomes and are useful for the investigation of hybrid genomes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00037014
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  • 4
    Digitale Medien
    Digitale Medien
    Analysis and chromosomal localization of retrotransposons in sugar beet (Beta vulgaris L.): LINEs andTy1-copia-like elements as major components of the genome (1995)
    Springer
    Chromosome research 3 (1995), S. 335-345 
    Details
    ISSN: 1573-6849
    Schlagwort(e): Beta vulgaris ; in situ hybridization ; LINE ; LTR retrotransposons ; non-LTR (non-viral) retrotransposons ; Ty1-copia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract DNA sequences of the reverse transcriptase gene of long terminal repeat (LTR) and non-LTR (non-viral) retrotransposons have been isolated and cloned from the genome of sugar beet (Beta vulgaris). Both retrotransposon types are highly amplified in sugar beet and may account for 2–5% of the genome. The BNR1 family, representing the first non-viral retrotransposon reported from a dicotyledonous species, shows homology to the mammalian L1 family of long interspersed repeated sequences (LINEs) and to retrotransposable elements from maize and lily. Sequences of the Tbv family are homologous to theTy1-copia class of LTR retrotransposons. The BNR1 and Tbv retrotransposon families are characterized by sequence heterogeneity and are probably defective. The deduced peptide sequences were used to investigate the relation to other retroelements from plants, insects and mammals. Fluorescencein situ hybridization was used to investigate the physical distribution and revealed that both retrotransposon families are present on all sugar beet chromosomes and largely excluded from chromosomal regions harbouring the 18S–5.8S–25S rRNA genes. The BNR1 family is organized in discrete clusters, while the Tbv family ofTy1-copia-like retrotransposons shows a more uniform distribution along chromosome arms and is absent from some chromosomal regions. These contrasting distributions emphasize the differences in evolutionary amplification and dispersion mechanisms between the two types of retrotransposons. Thein situ results of both elements reflect significant features of a higher order structure of the genome, as it is known for both short interspersed repeated sequences (SINEs) and LINEs in human.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00710014
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  • 5
    Digitale Medien
    Digitale Medien
    Molecular structure and chromosomal localization of major repetitive DNA families in the chickpea (Cicer arietinum L.) genome (1999)
    Springer
    Plant molecular biology 39 (1999), S. 1037-1050 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Cicer arietinum L. ; repetitive DNA ; retrotransposable element ; satellite DNA ; in situ hybridization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Three major repetitive DNA sequences were isolated from a genomic library of chickpea (Cicer arietinum L.) and characterized with respect to their genomic organization and chromosomal localization. All repetitive elements are genus-specific and mostly located in the AT-rich pericentric heterochromatin. Two families are organized as satellite DNAs with repeat lengths of 162–168 bp (CaSat1) and 100 bp (CaSat2). CaSat1 is mainly located adjacent to the 18S rDNA clusters on chromosomes A and B, whereas CaSat2 is a major component of the pericentric heterochromatin on all chromosomes. The high abundance of these sequences in closely related species of the genus Cicer as well as their variation in structure and copy number among the annual species provide useful tools for taxonomic studies. The retrotransposon-like sequences of the third family (CaRep) display a more complex organization and are represented by two independent sets of clones (CaRep1 and CaRep2) with homology to different regions of Ty3-gypsy-like retrotransposons. They are distributed over the pericentric heterochromatin block on all chromosomes with extensions into euchromatic regions. Conserved structures within different crossability groups of related Cicer species suggest independent amplification or transposition events during the evolution of the annual species of the genus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006125430386
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  • 6
    Digitale Medien
    Digitale Medien
    Detection of alien chromatin conferring resistance to the beet cyst nematode (Heterodera schachtii Schm.) in cultivated beet (Beta vulgaris L.) using in situ hybridization (1997)
    Springer
    Chromosome research 5 (1997), S. 186-193 
    Details
    ISSN: 1573-6849
    Schlagwort(e): Beta species ; marker-assisted selection ; molecular cytogenetics ; nematode resistance ; satellite DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Chromatin originating from wild beets of the genus Beta, section Procumbentes, has been investigated in nematode-resistant hybrid-derived lines of sugar beet (Beta vulgaris L.) by in situ hybridization using satellite, telomeric and ribosomal DNA repeats, a yeast artificial chromosome (YAC) and total genomic DNA as probes. The alien chromosome was detected in three monosomic addition lines(2n=18+1) by genomic in situ hybridization. Fluorescence in situ hybridization with a genome-specific satellite repeat and YAC DNA enabled the visualization of Procumbentes chromosomes, and in double-target hybridization it was shown that they do not carry 18S–5.8S–25S rRNA and 5S rRNA genes. The wild beet-specific satellite repeat and the telomere sequence from Arabidopsis thaliana were used to perform a structural analysis of the wild beet chromosome fragments of two resistant fragment addition lines. It was shown that one physical end of the chromosome fragments consists of telomeric repeats. Comparison of fragment sizes indicated that the small chromosome fragments harbouring the resistance gene most likely resulted from the loss of one wild beet chromosome arm and an internal deletion of the remaining arm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018447031020
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  • 7
    Digitale Medien
    Digitale Medien
    Beiträge zur Kenntnis von Hydrogelen. VII. Mitteilung. Über Chromioxydhydrate (1929)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 185 (1929), S. 107-129 
    Details
    ISSN: 0863-1786
    Schlagwort(e): Chemistry ; Inorganic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/zaac.19291850108
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  • 8
    Digitale Medien
    Digitale Medien
    Structure of an insect virus at 3.0 Å resolution (1987)
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 2 (1987), S. 167-176 
    Details
    ISSN: 0887-3585
    Schlagwort(e): icosahedral ; insect ; virus ; structure ; evolution ; nodavirus ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: We report the first atomic resolution structure of an insect virus determined by single crystal X-ray diffraction. Black beetle virus has a bipartite RNA genome encapsulated in a single particle. The capsid contains 180 protomers arranged on a T = 3 surface lattice. The quaternary organization of the protomers is similar to that observed in the T = 3 plant virus structures. The protomers consist of a basic, crystallographically disordered amino terminus (64 residues), a β-barrel as seen in other animal and plant virus subunits, an outer protrusion composed predominantly of β-sheet and formed by three large insertions between strands of the barrel, and a carboxy terminal domain composed of two distorted helices lying inside the shell. The outer surfaces of quasi-threefold related protomers form trigonal pyramidyl protrusions. A cleavage site, located 44 residues from the carboxy terminus, lies within the central cavity of the protein shell.The structural motif observed in BBV (a shell composed of 180 eight-stranded antiparallel β-barrels) is common to all nonstatellite spherical viruses whose structures have so far been solved. This highly conserved shell architecture suggests a common origin for the coat protein of spherical viruses, while the primitive genome structure of BBV suggests that this insect virus represents an early stage in the evolution of spherical viruses from cellular genes.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/prot.340020302
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  • 9
    Digitale Medien
    Digitale Medien
    Electronic and structural properties of defects in c-BN (1997)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 65 (1997), S. 941-946 
    Details
    ISSN: 0020-7608
    Schlagwort(e): Chemistry ; Theoretical, Physical and Computational Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: Using a molecular cluster with 71 atoms, we studied the electronic and structural properties of nitrogen vacancy, boron antisite, and oxygen in cubic boron nitride through an ab initio Hartree-Fock calculation. We found that all defects introduce a deep state above the middle-energy gap. These defects present a C3v local symmetry. In the case of nitrogen vacancy, the possibility of the F-center formation is discussed. All the calculations were performed using the program code GAMESS.   © 1997 John Wiley & Sons, Inc. Int J Quant Chem 65: 941-946, 1997
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    Übergangsmetall-substituierte Acylphosphane und Phosphaalkene. 17 [1] Synthese und Struktur der μ-Isophosphaalkin-Komplexe [(η5-C5H5)2(CO)2Fe2(μ-CO)(μ-C=PC6H2R3-2,4,6)] (R = Me, iPr, tBu) (1993)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 619 (1993), S. 1759-1764 
    Details
    ISSN: 0044-2313
    Schlagwort(e): Transition metal substituted phosphaalkenes ; isophosphaalkyne complex ; Chemistry ; Inorganic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Transition Metal Substituted Acylphosphanes and Phosphaalkenes. 17. Synthesis and Structure of the μ-Isophosphaalkyne Complexes [(η5-C5H5)2(CO)2Fe2(μ-CO)(μ-C=PC6H2R3)] (R = Me, iPr, tBu).Condensation of (η5-C5H5)2(CO)2Fe2(μ-CO)(μ-CSMe)}+SO3CF3- (6) with 2,4,6-R3C6H2PH(SiMe3) (7) (a: R = Me, b: R = iPr, c: R = tBu) affords the complexes (η5-C5H5)2(CO)2Fe2(μ-CO)(η-C=PC6H2R3-2,4,6) (9 a-c) with edge-bridging isophosphaalkyne ligands as confirmed by the x-ray structure analysis of 9 a.
    Notizen: Die Kondensation des μ-Carbinkomplexes {(η5-C5H5)2(CO)2Fe2(μ-CO)(μ-CSMe)}+SO3CF3- (6) mit den Phosphanen 2,4,6-R3C6H2PH(SiMe3) (7) (a: R = Me, b: R = iPr, c: R = tBu) liefert die Komplexe (η5-C5H5)2(CO)2Fe2(μ-CO)(η-C=PC6H2R3-2,4,6) 9a-c, die die neuartigen Isophosphaalkine C≡P-Aryl als Brückenliganden tragen. Von 9 a wurde eine Röntgenstrukturanalyse durchgeführt.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/zaac.19936191023
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