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  • 1
    Electronic Resource
    Electronic Resource
    Nonradioactive Northern blotting with biotinylated and digoxigenin-labeled RNA probes (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 1351-1355 
    Details
    ISSN: 0173-0835
    Keywords: Stripping ; Chemiluminescence ; Alkaline phosphatase ; Nonradioactive blotting ; Peroxidase ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The application of nonradioactive RNA probes for Northern blotting offers the advantage of a rapid turn-around time for results without the loss of sensitivity for target mRNA detection. However, a problem that has impeded the widespread use of nonradioactive RNA probes for use in Northern blotting is the difficulty in stripping these probes from nylon membranes after hybridization. In this report we describe two protocols for stripping digoxigenin (Dig)-labeled RNA probes from nylon membranes. One protocol utilizes a phosphate-buffered formamide stripping solution to remove nonchemically modified (regular) RNA probes while the other method utilizes strippable probes that were produced with a chemically modified nucleotide (CTP) and removed by a specific stripping solution. This latter method was developed by Ambion Inc. and is called Strip-EZ™. We also describe a protocol for the detection of two separate rat mRNAs using both biotin and digoxigenin-labeled RNA probes that does not require stripping the membrane after hybridization. Finally, we describe the use of another new labeling technology, called ChemLink™, that quickly and conveniently labels RNA for use in Northern blotting.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150190825
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  • 2
    Electronic Resource
    Electronic Resource
    Total Synthesis of Indole and Dihydroindole Alkaloids. IXFor a preliminary report on a portion of this work, see [1].Part VIII, see [2]. Studies on the synthesis of bisindole alkaloids in the vinblastine- vincristine series. The biogenetic approach (1976)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 59 (1976), S. 2858-2882 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A detailed study of the reaction of catharanthine N-oxide and vindoline has been carried out employing various conditions. Under optimum conditions, which involve low temperatures and trifluoroacetic anhydride as reagent, 3′, 4′-dehydrovinblastine (XIII, R = COOCH3), in reasonable yields is essentially the exclusive product. However two additional products, 18′ (epi)- 3′, 4′-dehydrovinblastine (XIV, R = COOCH3) and 1′-hydroxy- 3′, 4′-dehydrovinblastine (XVI, R = COOCH3) are also often isolated.The reaction, which follows the course of a Polonovski-type fragmentation process, has been extended to the N-oxide derivatives of dihydrocatharanthine and decarbomethoxycatharanthine to provide again a series of bisindole alkaloid derivatives, also vinblastines. A mechanistic rationale is provided to explain the various results obtained.
    Additional Material: 6 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19760590824
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  • 3
    Electronic Resource
    Electronic Resource
    Die Darstellung höherer Alkylrhodanide. (Nachtrag) (1940)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 156 (1940), S. 260-260 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19401560902
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  • 4
    Electronic Resource
    Electronic Resource
    Präparative und spektroskopische Untersuchungen an SilylperoxydenHerrn Professor Dr. W. Langenbeck zum 60. Geburtstage gewidmet (1959)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 8 (1959), S. 241-255 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Es werden spektroskopische Untersuchungen zur Strukturaufklärung von Siliciumperoxyden durchgeführt, einer neuen Verbindungsklasse, deren Erforschung im Rahmen der Entwicklung der peroxydischen Polymerisationskatalysatoren und Aktivatoren Bedeutung zukommt.Dabei wurden die IR- und Raman-Spektren der folgenden Präparate aufgenommen und untersucht: \documentclass{article}\pagestyle{empty}\begin{document}$$ \left({{\rm CH}_{\rm 3} } \right)_3 {\rm SiOOSi}\left({{\rm CH}_{\rm 3} } \right)_3,\left({{\rm CH}_{\rm 3} } \right)_3 {\rm SiOOC}\left({{\rm CH}_{\rm 3} } \right)_3,\left({{\rm CH}_{\rm 3} } \right)_3 {\rm COOC}\left({{\rm CH}_{\rm 3} } \right)_3,\left({{\rm C}_{\rm 2} {\rm H}_{\rm 5} } \right)_3 {\rm SiOOSi}\left({{\rm C}_{\rm 2} {\rm H}_{\rm 5} } \right)_3 $$\end{document} Es werden allgemeine Strukturbetrachtungen für Moleküle des Typs R3XOOXR3 als Viermassenmodell durchgeführt. Bei der Zuordnung der Spektren werden jeweils Vergleichssubstanzen herangezogen, die die Gruppierung R3X -  mit dem entsprechenden Peroxyd gemeinsam haben, um durch Aussonderung der Frequenzen des R3X-Gerüstes die Schwingungen der XOOX-Brücke isolieren zu können. Dabei kann als wahrscheinlichste Struktur für die Verbindungen des Typs R3SiOOSiR3 und R3COOCR3 ein wasserstoffperoxydähnlicher Bau der Moleküle abgeleitet werden. Diese Deutung wird durch die Tatsache gestützt, daß andere Bearbeiter mit Hilfe von Messungen elektrischer Momente zu ähnlichen Aussagen über den Molekülbau des Di-tert.-Butylperoxyds gelangen.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19590080501
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  • 5
    Electronic Resource
    Electronic Resource
    Alkalitrimethylsilanolate (1964)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 328 (1964), S. 105-112 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: From the reaction of trimethyl silanole with elementary rubidium and caesium, quantitative yields of the new compounds rubidium and caesium trimethylsilanolate have been obtained. Physical and chemical properties as well as IR and NMR spectra of the alkali trimethylsilanolates being now completed are reported and discussed.
    Notes: Durch Umsetzung von elementarem Rubidium und Caesium mit Trimethylsilanol wurden erstmals ein Rubidium- bzw. Caesiumtrimethylsilanolat in reiner Form dargestellt. Neben einer vergleichenden Betrachtung ihrer physikalischen und chemischen Eigenschaften wurden an den Verbindungen der auf diese Weise vervollständigten Reihe der Alkali-trimethylsilanolate IR- und NMR-spektroskopische Untersuchungen durchgeführt. Die Ergebnisse werden in Hinsicht auf die vorliegenden Struktur- und Bindungsverhältnisse diskutiert.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19643280110
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  • 6
    Electronic Resource
    Electronic Resource
    Some thiol-terminated polyethersJournal Series No. 462. (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 12 (1968), S. 107-113 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Thiol-terminated polyethers were prepared by two different routes, the starting materials being commercially available polyether polyols and epoxy resins. The products were pale colored liquids and were found to be very stable on storage at room temperature. Thiol-terminated polyethers of functionality from 2 to 4 were prepared. They are interesting building blocks for polymers through oxidation of the terminal SH groups or through coreaction with epoxy resins.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1968.070120109
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  • 7
    Electronic Resource
    Electronic Resource
    Über die Bestimmung der ungesättigten und aromatischen Anteile des Urteers (1923)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 36 (1923), S. 545-546 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19230367003
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  • 8
    Electronic Resource
    Electronic Resource
    Zur Frage der Zusammensetzung des Urteerbenzins (1923)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 36 (1923), S. 217-218 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19230363104
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  • 9
    Electronic Resource
    Electronic Resource
    Über die Bestimmung der aromatischen und ungesättigten Anteile im Urteer (1923)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 36 (1923), S. 266-267 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19230363905
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  • 10
    Electronic Resource
    Electronic Resource
    Über die Zusammensetzung des Schwelgases bei verschiedenen Temperaturintervallen des Drehofens (1924)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 37 (1924), S. 85-87 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19240370705
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