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  • 1
    Electronic Resource
    Electronic Resource
    Electrochemical evaluation of lactate dehydrogenase immobilized in polyacrylamide gels (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 19 (1977), S. 1785-1792 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Lactate dehydrogenase (EC 1.1.1.27) has been immobilized in polyacrylamide gels over a platinum grid matrix. The immobilized enzyme is used to oxidize L-lactate in the presence of nicotinamide adenine dinucleotide (NAD+) and femcyanide. The NADH produced is then chemically oxidized back to NAD+by ferricyanide. The coupled reduction of ferricyanide ions to ferrocyanide ions results in a measurable electrochemical potential. This measurable zero-current potential is found to be Nernstian in nature and directly proportional to the logarithm values of L-Iactate concentration over the range of 2 × 10-5to 5 × 10-2M. The results indicate that immobilized lactate dehydrogenase can be incorporated into a system to detect L-Lactate acid in aqueous solutions.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260191205
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  • 2
    Electronic Resource
    Electronic Resource
    Potentiometric method for subatrate analysis using immobilized NAD+-dependent oxidoreductase enzymes (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 1905-1915 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Two coenzyme-dependent oxidoreductases, glucose dehydrogenase and alcohol dehydrogenase, were immobilized in polyacrylamide gel over a platinum grid matrix and used as enzyme electrodes to measure their substrate concentrations in buffered aqueous solutions. The immobilized enzymes were used to oxidize their substrates in the presence of NAD+. Ferricyanide was used as the redox mediator and electroactive specific. The determinations of glucose and ethenol were utilized to demonstrate and evaluate the performance of the system. The described methodology should be readily applicable to the analysis of numerous other substrates of coenzyme-dependent oxidoreductases.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260211103
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 971-975 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10-4M to 10-1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260240416
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of mannosyl transferases during the pupal instar of Stomoxys calcitrans (L) (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 1 (1983), S. 1-15 
    Details
    ISSN: 0739-4462
    Keywords: Stomoxys calcitrans ; mannosyl transferase ; stable fly ; glycosyl transferase ; dolicholphosphate ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Particulate fractions (10,000g) from pupae of Stomoxys calcitrans transfer [14C]-mannose from GDP-[14C]-mannose to dolichol monophosphate and proteins. Production of the mannosyl lipid was inhibited by Mn2+, UDP, GMP, GDP, and EDTA. The insect growth regulator diflubenzuron had no effect on mannosyl transferase activity. Dolichol monophosphate and Mg2+ stimulated mannosyl transferase activity. The mannosyl lipid product was identified as mannosyl-phosphoryl-dolichol (Man-P-Dol). The apparent Km and Vmax values for the formation of Man-P-Dol using GDP-[14C]-Man while holding dolichol phosphate constant were 2.4 ± 0.9 μM and 9.4 ± 2.3 pmol Man-P-Dol·min-1·mg-1 protein, respectively. The apparent Km and Vmax values using dólichol phosphate while holding GDP-Man constant were 2.2 ± 1.2 μM and 18.5 ± 1.7 pmol Man-P-Dol·min-1·mg-1 protein.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/arch.940010102
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  • 5
    Electronic Resource
    Electronic Resource
    Solvent dependence of 13C relaxation times and reorientational dynamics in tribromobenzene (1988)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 26 (1988), S. 675-678 
    Details
    ISSN: 0749-1581
    Keywords: Tribromobenzene ; 13C spin-lattice relaxation times ; correlation times ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The 13C spin-lattice relaxation times and nuclear Overhauser enhancements of protonated carbons in 1, 3, 5-tribromobenzene were measured in a number of solvents of widely varying viscosity, Derived reorientational correlation times were found to increase approximately linearly with solution viscosity, as predicted by hydrodynamic theories of rotational diffusion.Rotational correlation times calculated from the perrin stick model were two to three times longer than the measured τc values. Similarly, correlation times predicted by the Hu-Zwanzig slip model were too small by a factor of two. On the other hand, application of the newer Hynes-Kapral-Weinberg theory furnished reorientational correlation times that were in virtually quantitative agreement with the experimental results.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260260807
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Raman and infrared study of reorientational dynamics in trifluorobenzene (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 20 (1989), S. 27-30 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The Raman spectral bandwidths of two A1′ vibrations of 1,3,5-trifluorobenzene (TFB) were measured in the neat liquid as a function of temperature and in the solvents CH2Cl2 and CS2. The Raman and IR linewidths of two E′ modes were also measured in solution. Derived values of the two rotational diffusion constants, D⊥ and D∥, were compared with the predictions of several current theories. It was observed that the Hynes-Kapral-Weinberg (HKW) theory is superior to both the microviscosity-free rotor and Hu-Zwanzig ‘slip’ models of reorientational diffusion. Calculated values of D⊥ and D∥ for TFB in solution using the HKW model are in excellent agreement with the experimentally measured diffusion constants.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250200106
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