ISSN:
1052-9306
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Simultaneous acylation/esterification of para-aminobenzoyl glutamic acid with trifluoroacetic anhydride and trifluoroethanol was attempted as part of a scheme to monitor deuterium-labeled dietary folates at nanogram levels in human subjects by GCMS. The anticipated fully-derivatized product comprised only about 10% of the reaction mixture; EI, positive ion CI and electron capture negative ion mass spectra of the major product (ca 80% yield) are consistent with a cyclic compound, most likely a lactam with a 5-membered ring. The stability and mass spectral characteristics of the cyclic compound permit differentiation of isotopically labeled species at subnanogram levels by selected ion monitoring in the electron capture negative ion mode.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200170203
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