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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 58 (1998), S. 329-332 
    ISSN: 0006-3592
    Keywords: tobacco cultured cells ; heat-shock promoter of Arabidopsis thaliana ; strong promoter from tobacco cell ; β-glucuronidase ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Construction of a gene expression system in tobacco cultured cells (BY2) was studied. A 925 bp promoter fragment of a heat-shock protein gene (HSP18.2) of Arabidopsis thaliana showed clear heat-shock response of expression of the β-glucuronidase (GUS) reporter gene in BY2 cells. Similar results were observed in a 500 mL flask and 3-L jar fermentor.Isolation of strong promoters in BY2 cells was tried. cDNA clones, in which the mRNA level is high in log-phase cells and the copy number in the genome is low, were isolated. These clones showed high homology with F1-ATPase (mitochondria type), elongation factor 1-α, and a gene with an unknown function of A. thaliana (clone 27), respectively. A 5′-flanking region of clone 27 showed 6.2 times the promoter activity of the CaMV35S promoter in BY2 cells.Three cDNA clones, which are expressed in the stationary growth phase of BY2 cells, were isolated by a differential screening. These clones showed high sequence homologies to alcohol dehydrogenase, pectin esterase, and extensin. Promoters of these genes will be useful in gene expression in high cell-density culture. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:329-332, 1998.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 33 (1987), S. 2657-2667 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: An emulsion polymerization of styrene in the presence of an amphoteric emulsifier of amino-acid type, N-lauroyl-N,N-dimethyllysine (DMLL), has been studied at pH 7.0. The polymerization rate increased exponentially with the concentration of DMLL. The concentration of latex particles was proportional to the DMLL concentration to the 0.6 power. The latex particle size decreased with increasing concentration of DMLL. The molecular weight of polystyrene latexes increased with an increase in DMLL concentration. Furthermore, the zeta potentials of latex particles were significantly dependent on the pH, and showed the existence of an isoelectric point at about pH 4.0.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 410 (1974), S. 313-315 
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Darstellung von T—Nb2O5-Einkristallen durch langsames Abkühlen unter hohem DruckNiob(V)-oxid in der T-Modifikation wurde bei 1800 K und 20 kbar in Gegenwart von H2O als Mineralisator in klaren Kristallen gewonnen. Das Präparat war frei von F, Cl und OH. Gitterkonstanten und d-Werte vgl. Tab. 1 und 2.
    Notes: The T form of niobium(V) oxide was prepared in clear crystals at 1800 K and 20 kbar in the presence of water as mineralizer. The sample was free of F, Cl, and OH. Lattice constants and X-ray data see table 1 and 2.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 28 (1994), S. 1365-1373 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: To produce a bone-bonding polymer surface that is capable of inducing deposition of a hydroxyapatite (HA) layer in the body fluid, an organophosphate polymer was covalently immobilized onto a high-density polyethylene film by surface graft polymerization of a phosphate-containing monomer. The grafted film was immersed in simulated physiologic solution (SPS). The chemical composition and structure of the formed apatite layer as well as its bonding strength to the polymer surface were investigated. To distinguish the effect of phosphate groups on the deposition of apatite layer from the simple calcium absorption by the anion, a comparative study was done using a polyethylene film with surface immobilized carboxylic groups. Calcium phosphate deposition was observed on all the materials investigated, but the kinetics, composition, deposit amount, and bonding strength of the new phase were found to be significantly different among the modified materials, depending on the density and chemical nature of the surface immobilized ionic groups. It was found that the polymeric materials modified by surface graft polymerization of a phosphate-containing monomer produce a carbonated HA layer firmly bonded with the material upon immersion in SPS. Carboxyl groups in the grafted layer was not enough to activate bonding with the HA layer. © 1994 John Wiley & Sons, Inc.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 24 (1996), S. 597-604 
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Surface modification and functionalization of polyaniline (PAN) and polypyrrole (PPY) films were achieved by Ar plasma treatment, near-UV-induced graft copolymerization with acrylic acid (AAc) and covalent immobilization of an enzyme (trypsin). The compositions and structures of the modified and functionalized polymer surfaces were characterized by angle-resolved XPS. Argon plasma treatment results in minor oxidations of the carbon atoms but is accompanied by a significant decrease in the intrinsic oxidation state ([=N-]/[-NH-] ratio) of the polymers. Graft copolymerization with AAc readily gives rise to self-protonated PAN and PPY surface structures. The surface concentration of the grafted AAc polymer increases with increasing monomer concentration and near-UV irradiation time during grafting but decreases with increasing plasma pretreatment time. Covalent immobilization of trypsin on the AAc-polymer-grafted PAN and PPY films is facilitated by the use of water-soluble carbodiimide intermediate (WSC). The effective enzyme activity increases initially with increasing surface concentration of the grafted AAc polymer, but becomes saturated at moderate to high AAc polymer concentration.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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