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  • 1
    Electronic Resource
    Electronic Resource
    Ethidium and azidoethidium oligonucleotide derivatives: Synthesis, complementary complex formation and sequence-specific photomodification of the single-stranded and double-stranded target oligo- and polynucleotides (1994)
    Chicester [u.a.] : Wiley-Blackwell
    Journal of Molecular Recognition 7 (1994), S. 177-188 
    Details
    ISSN: 0952-3499
    Keywords: Sequence-specific photomodification ; Oligonucleotide derivatives ; Ethidium ; Azidoethidium ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Oligodeoxyribonucleotide derivatives containing ethidium or azidoethidium residues attached to 3′ and/or 5′ end were prepared. These derivatives formed tight specific complexes with complementary oligodeoxyribonucleotides where each attached ethidium residue led to an increase of complex Tm by 20-30°C. Tandem complexes of two oligodeoxyribonucleotides containing ethidium residues with an oligodeoxyribonucleotide having two adjacent complementary sequences for these Oligonucleotide were investigated. Photoinduced reactions of a number of ethidium and azidoethidium oligodeoxyribonucleotide derivatives with target complementary single-stranded and double-stranded oligo- and polydeoxyribonucleotides were investigated. The irradiation led to direct photocleavage of the target oligo- or polynucleotide, to formation of hidden (piperidine cleavable) modifications of the target of formation of covalent adducts between ethidium oligodeoxyribonucleotide derivative and the target. In a number of experiments, azidoethidium dyes were demonstrated to be considerably stronger photosensitizers than ethidium ones. Depending on the nature of the target (single- or double-stranded DNA) and on the irradiation conditions, the total damages to the target oligo- or polydeoxyribonucleotides ranged from 10-70% (for ethidium dyes) to 30-80% (for azidoethidium dyes).
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmr.300070305
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  • 2
    Electronic Resource
    Electronic Resource
    Investigation of Stress-Strain Relations of initially anisotropic metals under proportional and nonproportional loading (1995)
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 26 (1995), S. 251-254 
    Details
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Untersuchung der Spannungs-Dehnungs-Beziehungen von anfänglich anisotropen Metallen unter den Bedingungen proportionaler und nicht proportionaler BelastungFür praktische Berechnungen basiert die Plastizitätstheorie üblicherweise auf der Annahme der Materialisotropie. Handelsübliche metallische Werkstoffe sind jedoch i.a. anisotrop infolge vorhergehender thermischer und/oder mechanischer Vorbehandlungen.Aluminium-, Titan- und Magnesiumlegierungen sind typische strukturanisotrope Werkstoffe. Deren breite Anwendung in vielen ingenieurtechnischen Bereichen erfordert jedoch eine genauere Bestimmung der mechanischen Eigenschaften unter verschiedenen typischen Belastungsbedingungen.In der vorliegenden Arbeit wird eine einfache Theorie für anisotrope Metalle vorgestellt, die auf Experimenten zur elastischplastischen Verformung ursprünglich anisotroper Metalle unter einfacher (proportionaler) Belastung sowie für den Spezialfall einer Belastung entlang eines unstetigen Spannungsweges beruht.
    Notes: In plasticity theories-applied in practical calculations- are usually based on the assumption of materials isotropy.Commercially obtained structural metallic materials however are originally anisotropic due to various kinds of thermal and mechanical treatments.Aluminium, titanium and magnesium alloys are typical anisotropic structural materials. The wide application of these materials in different fields of engineering demands more accurate mechanical behaviour predictions for these alloys under various conditions of loadings.In the aper, a simple theory of plasticity for anisotropic metals is proposed which is based on the results of experimental investigations into elasto-plastic deformation of initially anisotropic aluminium alloys subjected to simple (proportional) loading as well as under loading along stress path with a corner.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mawe.19950260508
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  • 3
    Electronic Resource
    Electronic Resource
    Site-specific laser modification (cleavage) of oligodeoxynucleotides (1989)
    New York : Wiley-Blackwell
    Biopolymers 28 (1989), S. 1129-1147 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Sequence-specific photomodification of oligodeoxynucleotide pAGAGTATTGACTTA (“a target”) has been carried out with the aid of complementary fluorescent probes. Such a probe consisted of oligodeoxynucleotide pAATACTCT and a chromophore group attached to its 5′ end. Three different derivatives of ethidium bromide were used as a chromophore. The photomodification was induced by nitrogen laser radiation (337 nm, 15 MW /cm2). The irradiation induces the following photodamages: target cleavage at the specific binding site with a cutting off of the 8-mer from its 5′ end (yield up to 12%), formation of specific covalent adduct target-probe with a yield of 20-70%, and piperidine-sensitive target modifications with a 7-27% yield (for different chromophores). The total yield of specific photodamages of all kinds is 50-80%. The target cleavage and generation of piperidine-sensitive modifications are optically nonlinear processes. Piperidine treatment of the irradiated samples led to specific cleavage of the target with the yield up to 40%. All kinds of observed modifications are not influenced by high concentrations of free radical scavengers: 1.3M tBuOH and 10 mM cystamine. The pattern of cleavage indicates that the most probable position of the chromophore is between T8 and G9 of the target, i.e., the chromophore stacks on top of the last A · T base pair of the duplex. The aggregate of evidence is in agreement with the mechanism of nonlinear photomodification (the cleavage and generation of piperidine-sensitive modifications) based on the transfer of two-photon excitation energy from the chromophore to the target.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360280607
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    Paper (German National Licenses)
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