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  • 1
    Electronic Resource
    Electronic Resource
    A production of monoclonal antibodies by a simple electrofusion technique induced by ac pulses (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 37 (1991), S. 790-794 
    Details
    ISSN: 0006-3592
    Keywords: Electrofusion ; AC pulses ; Monoclonal antibodies ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A simple and effective technique of electrofusion of the mouse myeloma cells and lymphocytes induced by AC pulses was investigated. Instead of pearl chain formation, we used a mild centrifugation of high-density cell suspensions to enhance the cell-cell contacts. The optimal frequency of the AC field used for cell fusion was 10 kHz. We confirmed the production of monoclonal antibodies against human serum albumin (HSA) by the hybridoma obtained by this method. Under the conditions used in the present work, the efficiency of hybridoma formation is several times higher than those obtained by the chemical method using polyethylene glycol (PEG). Hybridoma colonies were detected in as high as 95% of the wells and 33% of them proved positive against HSA.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260370814
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  • 2
    Electronic Resource
    Electronic Resource
    Application of glow discharge optical emission spectroscopy to diffusion measurement of copper in ferritic and austenitic iron and steels (1992)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 19 (1992), S. 638-642 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Glow discharge optical emission spectroscopy (GDOS) is a method to analyse the optical emission of elements during sputtering of a solid, providing data on the light intensities of the elements vs. the sputtering time. By correcting the measured light intensities with standard samples, it is possible to obtain the quantitative depth profile up to about 100 μm. In the present research, GDOS was applied to the diffusion measurement of impurity atoms in metals and alloys. After a thin film of copper was coated on iron and steels, the surface film was diffused into the matrix at high temperatures. The penetration curves of copper from the surface were obtained from the GDOS data. Diffusivities of copper in ferritic and austenitic regions of iron were determined from the penetration curves, which were in agreement with previous results. Diffusion of copper in commercial ferritic and austenitic stainless steels was also examined. The diffusivities obtained were comparable with those expected from the temperature dependence of the diffusivities in the ferritic and austenitic phases of pure iron.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.7401901119
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  • 3
    Electronic Resource
    Electronic Resource
    Quantitative GDOS analysis of oxide films on steels (1994)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 22 (1994), S. 134-138 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: A method for the quantitative analysis for oxide films on steels by means of glow discharge optical emission spectrometry (GDOS) was investigated. Effective optical emission yields for analysed elements were determined by measuring the emission intensities and sputtering rates of standard samples during discharging under constant conditions. The chemical composition in oxidized samples was obtained from the emission intensities and the effective emission yields, while the sputtered depth was calculated from the emission intensities and effective densities of elements. From measurements of the sputtering of magnetite the effective density for oxygen was estimated as 1820 kg m-3. It was shown that GDOS can be used to determine the quantitative composition and the thickness of the oxide layers on steels.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740220132
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  • 4
    Electronic Resource
    Electronic Resource
    Quantitative trace analysis by non-resonant laser post-ionization (1991)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 17 (1991), S. 773-778 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: In order to overcome the poor quantitativeness of conventional SIMS, sputtered neutral mass spectrometry (SNMS) employing non-resonant laser post-resonant laser post-ionization has been studied. By studied. By combining a time-of-flight (TOF) analyzer with a powerful excimer laser, it was confirmed that non-resonant laser post-ionization is capable of quantitative analysis of impurities at the pm level. On comparing laser wavelengths, 193 nm (ArF) gave a higher post-ionization efficiency than 248 nm (KrF). It was also demonstrated that the electronegative element sulphur can be detected as positive ions by post-ionization. Furthermore, surface metallic contaminants in a small area (250 μm square) could be determined down to 1011 atoms cm-2.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740171105
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  • 5
    Electronic Resource
    Electronic Resource
    Application of polymer films plasma polymerized in the tetramethylsilane/ammonia mixture to moisture sensor (1983)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Letters Edition 21 (1983), S. 353-357 
    Details
    ISSN: 0360-6384
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1983.130210507
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  • 6
    Electronic Resource
    Electronic Resource
    Capacitive moisture sensor devices from plasma films (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 31 (1986), S. 2473-2481 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Polymeric films were deposited on Al2O3 ceramic substrates by plasma deposition from trimethylsilyldimethylamine (TMSDMA) and bis(dimethylamino)methylvinylsilane (BDMAMVS), and their capacitance-relative humidity characteristics investigated. Films doped with methylbromide showed a linear dependence of log(capacitance) with relative humidity over the range 20-90% suggesting application on a moisture sensor device. Hysterisis effects were negligible and the response time was within 1 min.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1986.070310808
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  • 7
    Electronic Resource
    Electronic Resource
    Highly wettable films plasma-polymerized from ethylene oxide (1987)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 25 (1987), S. 1633-1639 
    Details
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Highly wettable films were prepared by plasma-polymerization from ethylene oxide. The surface energy of the formed films is 65.6 dyn/cm, and the contact-angle of water on the films is 12 degrees. The formation of highly wettable films is limited to the plasma-polymerization conducted at low W/FM values of below 200 MJ/kg. Analyses by infrared spectroscopy and ESCA show that the formed films contain much oxygen residues such as C—O, C—O, and C(O)—O— groups. Such polar groups would contribute high wettability of the plasma-films from ethylene oxide.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1987.080250616
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  • 8
    Electronic Resource
    Electronic Resource
    Synthese von Titanyl- und Vanadyl-mesoporphyrin-IX-dimethylester (1968)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 80 (1968), S. 914-915 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19680802116
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  • 9
    Electronic Resource
    Electronic Resource
    AES/GDS characterization of thin oxide films on Fe—Cr sheet steels (1991)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 17 (1991), S. 551-555 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Thin oxide films on four kinds of commercial sheet were characterized with Auger electron spectroscopy (AES) and glow discharge optical emission spectroscopy (GDS). Enrichment of alloying elements was noticed in the thin oxide films formed in air. The depth profiles obtained by AES with ion sputtering showed that enrichment of chromium occurred at the interface between the oxide and the matrix. It was difficult with AES to obtain the profiles of silicon and manganese because of overlap of their peaks with the iron peaks on the spectrum. On the other hand, with GDS, silicon and manganese could be analysed quantitatively in thin films that are 〈0.05 μm thick.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740170803
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  • 10
    Electronic Resource
    Electronic Resource
    Rate-enhancing effect of polyelectrolytes on interionic reactions. High-pressure influence on aquation of Co(NH3)5 Br2+ with various metal ions and role of dehydration (1976)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Letters Edition 14 (1976), S. 667-669 
    Details
    ISSN: 0360-6384
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1976.130141106
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