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  • 1
    Electronic Resource
    Electronic Resource
    Salicylaldehyde-metal-amino acid ternary complex: A new tool for immobilized metal affinity chromatography (1996)
    Chicester [u.a.] : Wiley-Blackwell
    Journal of Molecular Recognition 9 (1996), S. 375-382 
    Details
    ISSN: 0952-3499
    Keywords: IMAC ; garlic ; salicylaldehyde ; copper complexes ; amino acids ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: An immobilized salicylaldehyde (sal) was used to build various salicylaldehyde-copper-amino acid (Sal-Cu-AA) complexes which are stable at a range of pH values (2.0-11.0). The complexes were found to bind protein molecules as IMAC resins. Thirteen proteins were examined for their binding to a Sal-Cu-Gly column. The efficacy of the Sal-Cu-AA resin for protein separation were demonstrated by two examples. The first was a new purification process for garlic lectins from garlic crude extract. It seems that in this case the Sal-Cu-AA resins were more selective than IDA resin. The second was immobilization of concanavalin A (Con A) on the resin and using the immobilzed Con A for affinity chromatography of mannose-rich glycoprotein ovalbumin. The Con A could be later eluted with EDTA or imidazole and the Sal-containing polymer could be recharged again for further use.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Synthesis of poly-L-arginine (1966)
    New York : Wiley-Blackwell
    Biopolymers 4 (1966), S. 91-96 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Poly-L-arginine was prepared by the guanidization of poly-L-ornithine with 1-guanyl-3,5-dimethylpyrazole. The poly-L-ornithine was derived from poly-δ,N-trifluroacetyl-L-ornithine by removal of the blocking groups under mild basic conditions (1M piper-idine).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1966.360040110
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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