ISSN:
1615-6102
Keywords:
Chlamydomonas
;
Pyrenoid
;
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)
;
Stroma
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The pyrenoid is a protein complex in the chloroplast stroma of eukaryotic algae. After the treatment with mercury chloride, pyrenoids were isolated by sucrose density gradient centrifugation from cell-wall less mutant cells, CW-15, as well as wild type cells, C-9, of unicellular green algaChlamydomonas reinhardtii. Pyrenoids were characterized as a fraction whose protein/chlorophyll ratio was very high, and also examined by Nomarski differential interference microscopy. Most of the components consisted of 55 kDa and 16 kDa polypeptides (1∶1) which were immunologically identified as the large and small subunit of RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) protein, respectively. Some minor polypeptides were also detected. Substantial amount of RuBisCO protein is present as a particulate form in the pyrenoid in addition to the soluble form in algal chloroplast stroma.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01320276
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