ISSN:
1432-072X
Keywords:
Chromatium
;
Near infrared absorption spectra
;
Physiological and artificial spectral changes
;
Triton X-100
;
Subchromatophore fractions
;
Fluorescence emission spectra
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Spectral changes in the near infrared absorption region of chromatophores of Chromatium vinosum, strain D, were analyzed. Spectral changes dependent in growing cultures on temperature (33° C and 39° C, respectively) were compared to artificial changes which take place under the influence of the detergent Triton X-100. Like addition of Triton X-100 to chromatophores, transfer of cells from 39° C to 33° C leads to the reversible formation of an absorption band at 820 nm at the expense of a band at 850 nm. But in contrast to the influence of Triton X-100, chromatophores isolated from cells grown at 39° C (39° C chromatophores), contain on an identical bacteriochlorophyll basis, higher amounts of pigment absorbing at 880 nm and lower amounts of pigment absorbing at 800 nm than found in 33° C chromatophores. Triton X-100 treatment does not influence the production of light induced absorbance changes characteristic of photochemical reaction centers; it does, however, change absorption spectra of a subchromatophore fraction which does not exhibit the known light dependent reaction center responses. The spectral properties of this fraction are altered with Triton X-100 in a manner comparable to whole chromatophores, i.e. translocation of the 850 nm band to 820 nm. Subchromatophore fractions isolated from 33° C chromatophores, which display an absorption band at 820 nm show in the presence of menadione a light induced absorbance change at 835 nm. Subchromatophore fractions from 39° C chromatophores, which display no absorbance at 820 nm show a light induced adsorbance change at 835 nm only after addition of Triton X-100. Spectral changes in growing cultures are accompanied by changes in the fluorescence emission spectra of isolated chromatophores. While 33° C chromatophores exhibit a major fluorescence emission band at 929 nm plus a minor band at 890 nm 39° C chromatophores exhibit only one band at 937 nm.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00406082
Permalink
