ISSN:
1573-739X
Keywords:
Administration, continuous intraportal
;
Chromatography, high pressure liquid
;
Fluorouracil
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A rapid and sensitive high pressure liquid chromatographic method has been developed for the routine monitoring in serum of the antineoplastic agent fluorouracil, during continuous intraportal administration. Serum spiked with internal standard, bromouracil, was gently shaken for 10 min with ethylacetate at an acidic pH. The evaporated extract was dissolved in the mobile phase containing potassium phosphate buffer. Assays were performed at ambient temperature on a Chrompack LiChrosorb RP 18 column (2×100×3.0 mm) equipped with a guard column. The retention time of fluorouracil was 2–6 min. The calibration curve was linear from 25 to 2,000 ng/ml. The coefficient of variation was ≤1.6% for within-run precision and ≤2.9% for day-to-day precision. The mean recovery of fluorouracil was 57%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01970157
Permalink
