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  • 1
    ISSN: 1432-5233
    Keywords: Apolipoprotein B-48 ; Triglyceride-rich lipoproteins ; Chylomicrons ; Hypertriglyceridaemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have previously demonstrated alterations in apolipoprotein B-48 metabolism in the post-prandial state in patients with non-insulin-dependent diabetes mellitus. This study investigates the relationship between hypertriglyceridaemia and post-prandial lipoprotein metabolism. Four groups of patients were examined: non-insulin-dependent diabetic patients, with normal serum triglyceride levels (serum triglyceride 〈2.1 mmol l−1; haemoglobin HbA1c 5.5%±0.4%); poorly controlled, non-insulin-dependent diabetic patients with hypertriglyceridaemia (serum triglyceride 〉2.1 mmol 1−1; HbA1c 8.8%±0.9%); nondiabetic subjects with serum triglycerides 〈2.1 mmoll−1; and non-diabetic subjects with hypertriglyceridaemia (serum triglyceride〉2.1 mmol l−1). Subjects were studied fasting and following a high-fat meal (1300 kcal). The triglyceride-rich lipoprotein fraction was isolated by ultracentrifugation (d〈1.006 g ml−1). Apoprotein B-48, apoprotein B-100 and apoprotein E were separated on 4%–15% gradient gels and quantified as a percentage of the fasting concentration by densitometric scanning. Triglyceride-rich lipoprotein apolipoprotein B-48 and apolipoprotein B-100 post-prandial profiles demonstrated a maximum increase either at 2 h or rising still further to a peak at 6 h before falling in the diabetic groups and hypertriglyceridaemic non-diabetic subjects when compared with the normotriglyceridaemic control subjects whose levels decreased after 2 h (P〈0.05). A significantly different triglyceride-rich lipoprotein apolipoprotein E profile was also exhibited by the diabetic patients (P〈0.05). Levels of triglyceride-rich lipoprotein, cholesterol, triglyceride, total protein and apoprotein B were elevated in the hypertriglyceridaemic subjects, both diabetic and non-diabetic. These results indicate that hypertriglyceridaemia is associated with altered metabolism and composition of post-prandial triglyceride-rich lipoprotein particles in both poorly controlled diabetic and non-diabetic subjects.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 23 (1996), S. 1241-1262 
    ISSN: 0271-2091
    Keywords: axisymmetric flow ; vortex method ; laminar flow ; entrance length ; steady and oscillating flows ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Steady and oscillating axisymmetric tube flows are modelled using a vorticity transport algorithm. The axisymmetric convective -diffusive Navier-Stokes equations are solved using a splitting technique. Axisymmetric ring vortex filaments are introduced on the walls and subsequently convected and diffused throughout the flow field. An axisymmetric equation similar to the Oseen diffusion equation is used to diffuse the ring vortex filaments. Vorticity is reflected from the tube walls using two techniques. Results are presented for the developing Poiseuille flow and for the developed flow in the form of the entrance length and the axial velocity and vorticity profiles. Good agreement is achieved with a finite difference method in the developing region of Poiseuille flow. The developed flow results are compared with the analytical solutions. The developed profiles of velocity and vorticity have errors of less than 0ċ3 per cent for both methods of dealing with reflection of diffusion at the bounding surfaces and similar accuracy is obtained for the velocity profiles in oscillating flow except at the wall. Oscillating flow is produced with a discretized sinusoidal piston motion. Velocity profiles, boundary layer thickness and entrance length are presented for oscillating flow. Good agreement is achieved for low-Womersley-number non-dimensional frequency. At higher values of this parameter, flows are inaccurately simulated, because the number of piston positions used to discretize the piston motion is inversely proportional to the non-dimensional frequency.
    Additional Material: 19 Ill.
    Type of Medium: Electronic Resource
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