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  • 1
    Electronic Resource
    Electronic Resource
    Male-sterile chicory cybrids obtained by intergeneric protoplast fusion (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 347-352 
    Details
    ISSN: 1432-2242
    Keywords: Cichorium intybus ; Protoplast fusion ; Cytoplasmic male sterility ; Mitochondrial DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Male-sterile chicory plants were obtained by fusion of chicory mesophyll protoplasts and hypocotyl protoplasts derived from male-sterile sunflower plants. The protoplasts of both species were fused by the PEG method and the products were selected manually and cultivated at very low density in a liquid medium. Three to twenty percent of the heterokaryocytes divided and evolved into microcalli, then into calli where budding could be induced. The mitochondrial genome of ten male-sterile or totally sterile plants was studied. Restriction endonuclease profiles of mitochondrial DNA and molecular hybridization with specific genes of the mitochondrial genome used as probes indicated that mitochondrial DNA rearrangement had occurred between sunflower and chicory and the intensity of the rearrangements correlated with the degree of sterility of the different plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01184922
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The induction of tetraploidy in chicory (Cichorium intybus L. var. Magdebourg) by protoplast fusion (1992)
    Springer
    Euphytica 62 (1992), S. 63-67 
    Details
    ISSN: 1573-5060
    Keywords: chicory ; Cichorium intybus ; protoplast fusion ; tetraploids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fusions of chicory leaf protoplasts were realized in order to obtain a higher number of tetraploid plants compared to the traditional doubling techniques with colchicine. Thanks to the fusion technique, using a mixture of PEG, DMSO and a solution of CaCl2 (pH 10.5), 25% tetraploid (2n=36) plants were obtained. Among the 167 regenerated plants, only one aneuploid of a near tetraploid level (2n=33) was identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036089
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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