ISSN:
1432-0983
Keywords:
Key wordsPichia anomala
;
Invertase
;
Cloning
;
Sequence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A genomic library from the yeast Pichia anomala has been constructed and employed to clone the gene encoding the sucrose-hydrolysing enzyme invertase by complementation of a sucrose non-fermenting mutant of Saccharomyces cerevisiae. The cloned gene, INV1, was sequenced and found to encode a polypeptide of 550 amino acids which contained a 22 amino-acid signal sequence and ten potential glycosylation sites. The amino-acid sequence shows significant identity with other yeast invertases and also with Kluyveromyces marxianus inulinase, a yeast β-fructofuranosidase which has a different substrate specificity. The nucleotide sequences of the 5′ and 3′ non-coding regions were found to contain several consensus motifs probably involved in the initiation and termination of gene transcription.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050041
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