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  • Artikel: DFG Deutsche Nationallizenzen  (3)
  • Cereals  (2)
  • Cohort studies  (1)
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  • Artikel: DFG Deutsche Nationallizenzen  (3)
Materialart
Erscheinungszeitraum
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Current genetics 22 (1992), S. 101-105 
    ISSN: 1432-0983
    Schlagwort(e): Intergenic region ; Stem rusts ; Puccinia graminis ; Leaf rusts ; Cereals
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Total genomic DNA was isolated from three cereal stem rusts, Puccinia graminis f. sp. tritici, f. sp. secalis, f. sp. avenae, and two cereal leaf rusts, P. recondita f. sp. tritici and P. coronata f. sp. avenae, and analyzed for the presence of heterogeneity in the intergenic region of the ribosomal DNA repeat unit. A 1 kb region of the repeat unit between the 26s and the 5s rRNA genes (IGR-1) was amplified by PCR and was found to be heterogeneous within each isolate and variable in size between races and species. The PCR results were confirmed by Southern blot analysis of native DNA. In an isolate of race C36(48), heterogeneity appeared to be due to variable numbers of 0.1 kb subrepeats in IGR-1. Nine wheat stem rust strains representing nine different races produced a unique pattern of heterogeneity while two different isolates of one race were identical, as were five of another. This may provide a rapid method for race identification in wheat stem rust. Heterogeneity and polymorphism in rye stem rust, oat stem rust, wheat leaf rust, and oat crown rust, was less pronounced than in wheat stem rust. In the course of this work, the 5s rRNA gene was located and its position and orientation within the ribosomal repeat unit was established.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Current genetics 15 (1989), S. 161-166 
    ISSN: 1432-0983
    Schlagwort(e): Double-stranded RNA ; dsRNA ; Mitochondria ; Stem rusts ; Leaf rusts ; Cereals
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Double-stranded ribonucleic acids (dsRNA) were isolated from mitochondria in urediosporelings of three cereal stem rusts, Puccinia graminis f. sp. tritici, f. sp. secalis and f. sp. avenae, and two cereal leaf rusts, P. recondita f. sp. tritici and P. coronata f. sp. avenae, and analyzed by agarose gel electrophoresis. The double strandedness of the RNA molecules was characterized by nuclease treatments (RNase A, DNase 1 and S1 nuclease) and CF-11 cellulose column chromatography. No interspecific variation in multisegments of dsRNA was observed among races of each forma specialis. As to the interspecific variation, although each of three forma specialis of Puccinia graminis had similar dsRNA segments, 4.8, 5.0 and 5.2 kb, wheat leaf rust and oat crown rust had additional dsRNA segments of 2.7, 2.8, 5.8 and 6.0 kb. The presence of a dsRNA segment of 5 kb size in all isolates and species examined indicates that this unique segment can be a molecular marker for the rust family, Uredinales. Dot-blot hybridization indicated that there is no sequence homology between dsRNA segments and mitochondrial DNA.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    International archives of occupational and environmental health 68 (1996), S. 277-288 
    ISSN: 1432-1246
    Schlagwort(e): Petroleum refinery workers ; Cohort studies ; Occupational mortality
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract An update of a cohort study of 4855 employees at a Paulsboro, New Jersey refinery was conducted to further examine mortality patterns. The earlier study investigated refinery workers employed for a minimum of 1 year between 1 January 1946 and 1 January 1979. The vital status of these workers was ascertained through 1979. The update extended enrollment in the study and vital status follow-up for an additional 8 years (1980–1987). As in the previous study, mortality from all causes [standardized mortality ratio (SMR) = 87; 95% confidence interval (95% CI): 83–91] was significantly lower than expected compared with the general population. Total cancer mortality was also lower than expected (SMR = 96; 95% CI: 86–106). A borderline significant mortality increase in prostatic cancer was found (SMR = 144; 95% CI: 106–190). This increase was similar to the nonsignificant increase reported in the original study (SMR = 135; 95% CI: 90–196). The excess was of comparable magnitude among white males and nonwhite males, although it was not significant for the latter. Detailed analysis indicated that the prostatic cancer was not likely to be related to employment at the refinery. Mortality from lymphatic and hematopoietic cancers was similar to the expected mortality. Mortality from overall leukemia was as expected and detailed analyses by specific cell type showed no increase. An increase in mortality occurred from non-Hodgkin's lymphoma among male workers (SMR = 132; 95% CI: 74–217). The increase was not statistically significant and unlikely to be associated with refinery employment. Mortality from multiple myeloma among male employees was lower than expected (SMR = 74; 95% CI: 20–190). Mortality from asbestos-related diseases (pulmonary fibrosis, lung cancer, malignant mesothelioma) was also lower than expected among male workers. No cause-specific mortality was found to be associated with duration of employment at the refinery, including several causes which have been reported to be elevated in previous studies. The findings of this updated study indicate, as in the previous report, the generally favorable mortality experience of Paulsboro refinery workers.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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