ISSN:
1573-5044
Keywords:
Coleus blumei (suspension cultures)
;
hydroxycinnamic acid esters
;
phenylalanine
;
rosmarinic acid
;
secondary metabolism
;
tyrosine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Suspension cultures of Coleus blumei accumulate very high amounts of rosmarinic acid, an ester of caffeic acid and 3,4-dihydroxyphenyllactate, in medium with elevated sucrose concentrations. Since the synthesis of this high level of rosmarinic acid occurs in only five days of the culture period, the activities of the enzymes involved in the biosynthesis are very high. Therefore all the enzymes necessary for the formation of rosmarinic acid from the precursors phenylalanine and tyrosine could be isolated from cell cultures of Coleus blumei: phenylalanine ammonia-lyase, cinnamic acid 4-hydroxylase, hydroxycinnamoyl:CoA ligase, tyrosine aminotransferase, hydroxyphenylpyruvate reductase, rosmarinic acid synthase and two microsomal 3- and 3′-hydroxylases. The main characteristics of these enzymes of the proposed biosynthetic pathway of rosmarinic acid will be described.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00033875
Permalink
