ISSN:
0003-276X
Keywords:
Lymph node
;
Colloidal gold
;
X-ray
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
Background: Tracer studies are a important tool to obtain information about the processes involved in the immunological response. Colloidal gold is widely used as a tracer, but its small size of label can cause some difficulty during low-resolution analysis. To overcome this difficulty, se developed a new method to follow the route of tracer movement within lymph nodes.Methods: We applied conventional X-ray analysis, X-ray flurescence analysis (XFA) subtractional microscop using synchrotron radiation (SR) beam, light mycroscopy, and ultrastructural analysis to study the distribution and quantity of colloidal gold coupled with albumin with rat parasternal lymph node 2, 4, 6, 8, and 10 h after intrapleural injection of the tracer.Results: At all the time points XFA-SR revealed that racer formed a circle with a maximum concentration in the node periphery. XFA-SR measured colloidal gold concentration in the nodes reached its maximum (0.5-0.75 weight%) in 6-8 h. Substractional microscopy revealed superficially located groups of cells filled with colloidal gold tracer. Light microscopy and ultrastructural analysis confirmed that the tracer was concentrated in the reticular cells, situated in the sinuses of the node. Sinusoidal reticular cells concentrated tracer at much higher rates than sinusoidal macrophages. Four hours after injection, gold appeared in the lysosmes of the follicular reticular cells. At the same time point, evidence of antigen presentation was obtained. Antigen presentation proved to be an extremel sentation was obtained. Antigen presentation proved to be an extremely rare event since only one ultrastructural incident was found in 150 analysed grids.Conclusion: SR is a valuable tool for the analysis of gold tracer passage with in the living organism. © 1995 Wiley-Liss, Inc.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1092410205
Permalink
