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  • Column liquid chromatography  (2)
  • shade trees  (2)
  • 1
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Vitamin B and C in tablets ; Experimental design ; Optimization of separation ; Multiple regression modeling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The ion-pair liquid-chromatographic separation of the water-soluble vitamins thiamine mononitrate, riboflavin phosphate, nicotinamide, pyridoxine hydrochloride, ascorbic acid, saccharin, and the dye Sunset Yellow FCF (E110) has been examined for the analysis of the compounds in effervescent tablets. A Draper-Lin small composite design was used to study the impact on the compounds' retention times and peak-widths (the response variables) of four different mobile phase variables: percentage of methanol as organic modifier, the concentrations of hexanesulfonate as ion pairing reagent and of triethanolamine as competitive base, and pH. The interactions of these variables were also studied. The proposed design enabled derivation of multiple linear regression equations to model each compound's retention time and peak-width at half-height. The statistical reliability of the regression models was established by comparing predicted and experimental values. By introducing the regression models into a spreadsheet program (Excel 5.0), retention times and peak-widths for each compound were calculated at fixed mobile phase pH. The values of all the other combinations of the three mobile phase variables were changed in increments of two units within their examined boundaries, resulting in 225 different rows. For each combination the compounds' calculated retention times and their corresponding peak-widths were sorted in increasing order and the resolution between successive peaks was calculated. The minimum effective resolution (R s min) between each pair of peaks and the maximum retention time (t R max) in each row were then selected and used to construct contour plots indicating the location of zones of mobile phase parameter combinations whereR s min〉1.5 and the analysis was rugged, and wheret R max values were minimum. Their common regions resulted in optimum chromatographic separations. Examples are shown of chromatographic separations obtained using mobile phase combinations which were the best compromise of the three criteria selected.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Optimization ; Mixture design ; Special cubic polynomial model ; Pareto-optimality ; Overlapping resolution mapping (ORM)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A systematic approach, using a mixture-design statistical technique, has been developed for selecting the optimum mobile phase for the separation of fat-soluble vitamins in reversed-phase high-performance liquid chromatography. A quaternary mixture of methanol, acetonitrile, tetrahydrofuran and water was used as mobile phase. Retention time and peak width were recorded in ten runs augmented with five replicates and the data were subsequently fitted to special cubic polynomial models. The resulting mathematical equations enabled prediction of resolution over the entire parameter space. Contour plots of minimum effective resolution and maximum retention time as a function of mobile phase composition are presented and discussed. Visual inspection of these plots provides an overview of the quality of the separation and the analysis time required for each possible mobile-phase composition with n the parameter space. It is demonstrated that the methodology followed was an important tool which enabled the taking of informed decisions necessary for selection of the optimum mobile phase for a chromatographic separation. A combination ofR S minimum andt R maximum as optimization criteria in a multicriteria decision-making plot using pareto-optimality concept is discussed. This combination enabled visual demonstration of the compromise between separation quality and the economics of analysis time. Our methodology has been compared with the common used technique of ‘overlapping resolution mapping’.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1572-9680
    Keywords: Ca ; Cordia alliodora ; Erythrina poeppigiana ; evaporation ; interception ; K ; Mg ; N ; P ; shade trees ; Theobroma cacao ; transpiration ; water balances
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The hydrological balances for the agroforestry systems of Theobroma cacao with Cordia alliodora or Erythrina poeppigiana were calculated using measurements taken over four years (1983–1987) of the following parameters: climatic (precipitation, evaporation); edaphological (texture, soil moisture tension, hydraulic conductivity, variations in soil moisture content). Water fluxes (interception, simulation of transpiration and percolation) were estimated from one year of measurements (1986–1987). Weekly samples of percolated water were taken from March 1986 – March 1987 using lysimetric capsules at 100 cm soil depth. N, P, K, Ca and Mg concentrations were determined to calculate the annual leaching losses. Nutrient concentration values were extrapolated for the whole 4 years observation period, in order to calculate leaching losses for the whole study period. Precipitation samples were also taken to determine the corresponding annual nutrient inputs.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1572-9680
    Keywords: Agroforestry ; biomass ; cocoa yield ; Cordia alliodora ; Erythrina poeppigiana ; litter production ; organic material ; shade trees ; sustainability ; timber production ; Theobroma cacao
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Predictive models were developed for Cordia alliodora branch and Theobroma cacao branch or leaf biomass,based on branch basal areas (r2 ⩾ 0.79) but the model of C. alliodora leaf biomass, although significant, was of very low accuracy (r2 = 0.09) due to annual leaf fall. At age 10 years, shade tree stem biomass accounted for 80% of the total above-ground biomass of either tree. However, between the ages of 6 and 10 years, the biomass increment of T. cacao branches (3–4t.ha−1.a−1) was similar to that of the shade tree stems. During the same period, the net primary productivity was 35 and 28 t.ha−1.a−1, for the Erythrina poepigiana and and C. alliodora systems, respectively. Cocoa production under either of the shade trees C. alliodora or E. poeppigiana was 1000 kg.ha−1.a−1 (oven-dry; ages 6–10 yr). During the same period, C. alliodora timber production was 9 m3.ha−1.a−1 whilst the leguminous shade tree E. poeppigiana does not produce timber. Litterfall over the same 5 years, including crop and/or shade tree pruning residues, averages 11 and 23 t.ha−1.a−1, respectively. The main difference was due to E. poeppigiana pruning residues (10t.ha−1.a−1). Soil organic material reserves (0–45 cm) increased over 10 years from 198 to 240 t.ha−1 in the E. poeppigiana plots and from 168–184 t.ha−1 in the C. alliodora plots. These values, together with the productivity indices presented, provide evidence that the systems are sustainable. For economic reasons, the use of C. alliodora is recommended under the experimental conditions. however, on less fertile soils without fertilization, the greater biomass and hence nutrient return to the soil surface under E. poeppigiana, might make this the preferable shade tree.
    Type of Medium: Electronic Resource
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