ISSN:
0935-6304
Keywords:
Liquid chromatography, HPLC
;
Column switching
;
Step gradient
;
Immunosuppressive agent
;
Determination in blood and plasma
;
Deproteinization
;
On-line sample clean-up
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A method for the determination of cyclosporin A in human whole blood and plasma is described which uses liquid chromatography with step gradient elution and a column switching technique. The chromatographic conditions chosen allow simple and rapid sample preparation, so that a result can be obtained within one hour. Blood and plasma are deproteinized with diluted methanol and an aliquot of the clear supernatant is directly injected. The detection limit for cyclosporin A is about 20 ng/ml starting from a 0.5 ml sample. The method is sensitive enough for monitoring the drug in the therapeutic range.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240050806
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