ISSN:
1432-1335
Keywords:
TNF
;
Microtubule effectors
;
Combination
;
Synergism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Combinations of human recombinant tumour necrosis factorα (rhTNFα) with each of four different agents disturbing the microtubule system of the cellular cytoskeleton were tested for synergistic cytotoxic action against murine melanoma B16K and L-M(S) cells. In addition to the known microtubule effectors colchicine, vincristine, and taxol, the influence of the fluorenone-azomethine derivativeα-diphenylene-N-{p-[bis-(Β-hydroxyethyl)-amino]-phenyl}-nitrone (DHPN) on the rhTNFα cytotoxicity was studied. Applying a novel computerbased isobole method [Suehnel J (1990) Antiviral Res 13:23–40] concentration ranges of synergistic, zero, and antagonistic interaction were found after in vitro combination of rhTNFα with each of the drugs tested in a 72-h cytotoxicity assay. In contrast, a 24-h exposure of B16K cells to these combinations still did not inhibit in vitro colony formation to a greater extent than either drug alone. A preliminary in vivo experiment revealed an increased antitumour effect after treatment of established subcutaneous melanoma B16 tumours with a combination of rhTNFα and DHPN.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01625431
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