ISSN:
1617-4623
Keywords:
Biotin
;
Arabidopsis
;
Complementation
;
bioA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract TheArabidopsis thaliana biotin auxotrophbio1 was rendered prototrophic by transformation with a chimeric transgene containing theEscherichia coli bioA gene driven by a constitutive promoter. ThebioA gene encodes the biotin biosynthetic enzyme 7,8-diaminopelargonic acid aminotransferase. Unlike the untransformed control plants, transgenic plants expressing the bacterial transgene synthesized biotin and grew to maturity without biotin-deficiency symptoms. These findings demonstrate thatbio1/bio1 mutant plants are defective in the gene encoding 7,8-diaminopelargonic acid aminotransferase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02172516
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