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  • 1
    ISSN: 1432-0983
    Keywords: Cosmid ; Positive selection ; Insert stability ; Gene banks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe the construction of a cosmid cloning vector, pMT555, which allows positive selection for the presence of an inserted DNA fragment. The vector contains sequences which enable its replication and selection in either E. coli or Saccharomyces cerevisiae. We demonstrate that pMT555 may be used for the efficient construction of total genomic banks from small quantities of donor DNA. The positive selection permits the stable maintenance of the cosmid in E. coli and the faithful replication of inserted sequences.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Monatsschrift Kinderheilkunde 147 (1999), S. 921-923 
    ISSN: 1433-0474
    Keywords: Schlüsselwörter Aseptische Gonarthritis ; Synovitis ; Kniegelenksonographie ; Agammaglobulinämie ; Mykoplasmeninfektion ; Key words Sterile gonarthritis ; Synovitis ; Ultrasonography of the knee ; Agammaglobulinemia ; Mycoplasma infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A non-traumatic and sterile effusion of the knee joint was the presenting symptom of agammaglobulinemia in a five year old boy. The patient had experienced recurrent infections, but these had been localized and responded to antibiotics so that the disease had not been diagnosed before. Synovitis of the knee was demonstrated by ultrasonography, while HRCT of the lungs showed thickening and dilatation of bronchial walls. Complete recovery of gonarthritis was achieved following intravenous gamma globulin substitution. Discussion: Agammaglobulinemia has to be considered in the presence of an atraumatic and sterile monarthritis of the knee. Ultrasonography is a valuable diagnostic tool in demonstrating synovitis.
    Notes: Zusammenfassung Ein fünfjähriger Junge entwickelte einen einseitigen nicht traumatischen, aseptischen Kniegelenkerguß, der sonographisch eine ausgeprägte Synovialishyperplasie zeigte. Die weitere Diagnostik erbrachte eine Agammaglobulinämie sowie Bronchiektasen. Eine vollständige und bleibende Remission der Kniegelenkbeschwerden wurde nach i.v. Immunglobulinsubstitution erreicht. Diskussion: Bei einer aseptischen Arthritis sollte auch an einen Immunglobulinmangel gedacht werden. Die Synovitis kann sonographisch gut dargestellt werden.
    Type of Medium: Electronic Resource
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