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  • 1
    Electronic Resource
    Electronic Resource
    The nature of callose produced during self-pollination inSecale cereale (1980)
    Springer
    Planta 148 (1980), S. 498-509 
    Details
    ISSN: 1432-2048
    Keywords: Callose ; Pollination ; Secale ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response to incompatible (self) pollination in rye (Secale cereale L.) includes the rapid deposition in the germinating pollen grain and pollen tube of a substance that stains with aniline blue, resorcin blue and calcofluor, and in these respects resembles callose. This substance has been isolated and analysed by acid hydrolysis and methylation as well as specific enzyme hydrolysis. It contains a glucan component with 1,4-β-glucosidic and 1,3-β-glucosidic linkages within the same linear chains. The proportion of 1,4-to 1,3-glucosidic linkages in the preparation is 77∶9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395321
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Composition of the cell walls of Nicotiana alata Link et Otto pollen tubes (1985)
    Springer
    Planta 166 (1985), S. 128-133 
    Details
    ISSN: 1432-2048
    Keywords: Arabinan ; Callose ; Cell wall (pollen) ; Nicotiana (Pollen) ; Pollen tube (wall)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell walls isolated from pollen of Nicotiana alata germinated in vitro contain glucose and arabinose as the predominant monosaccharides. Methylation analysis and cytochemical studies are consistent with the major polysaccharides being a (1→3)-β-D-glucan (callose) and an arabinan together with small amounts of cellulose. The cell walls contain 2.8% uronic acids. Alcian blue stains the pollen-tube walls intensely at the tip, indicating that acidic polysaccharides are concentrated in the tip. Synthetic aniline-blue fluorochrome is specific primarily for (1→3)-β-D-glucans and stains the pollen-tube walls, except at the tip. Protein (1.5%), containing hydroxyproline (2.4%), is present in the cell wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397395
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The nature of callose produced during self-pollination inSecale cereale (1980)
    Springer
    Planta 148 (1980), S. 498-509 
    Details
    ISSN: 1432-2048
    Keywords: Callose ; Pollination ; Secale ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response to incompatible (self) pollination in rye (Secale cereale L.) includes the rapid deposition in the germinating pollen grain and pollen tube of a substance that stains with aniline blue, resorcin blue and calcofluor, and in these respects resembles callose. This substance has been isolated and analysed by acid hydrolysis and methylation as well as specific enzyme hydrolysis. It contains a glucan component with 1,4-β-glucosidic and 1,3-β-glucosidic linkages within the same linear chains. The proportion of 1,4-to 1,3-glucosidic linkages in the preparation is 77∶9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552666
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    The cryopreservation ofChlorella (1978)
    Springer
    Archives of microbiology 119 (1978), S. 153-156 
    Details
    ISSN: 1432-072X
    Keywords: Chlorella ; Cryopreservation ; Fatty acids analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Following growth under sub-optimal concentrations of nutrients, cells ofChlorella emersonii accumulated lipid and became more resistant to the damage caused by freezing and thawing. These results suggest that the factor responsible for the cold hardening of someChlorella spp is not the effect of low temperatures per se but simply that of the reduced metabolic rate. Evidence is given that the post-thaw injury observed following rapid rates of cooling is associated with the vacuole.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00964266
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    The cryopreservation of Chlorella (1977)
    Springer
    Archives of microbiology 114 (1977), S. 249-254 
    Details
    ISSN: 1432-072X
    Keywords: Chlorella ; Cryopreservation ; Fatty acid analysis ; Preeze-fracture electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Following a shift from autotrophic to heterotrophic nutrition, cells of Chlorella protothecoides become sensitive to the stresses of freezing and thawing. The injury then observed at slow rates of cooling cannot be explained by the cellular response to hypertonic solutions, and at faster cooling rates intracellular ice formation was not demonstrated to be damaging. These findings are at variance with suggested mechanisms of injury in other cellular systems. The results are compared with alterations in ultrastructure and in the composition of the cellular fatty acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446869
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    The location of (1→3)-β-glucans in the walls of pollen tubes of Nicotiana alata using a (1→3)-β-glucan-specific monoclonal antibody (1991)
    Springer
    Planta 185 (1991), S. 1-8 
    Details
    ISSN: 1432-2048
    Keywords: Callose ; Cell wall ; (1→3)-β-Glucan ; Nicotiana alata ; Pollen tube ; Monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The location of the (1→3)-β-glucan, callose, in the walls of pollen tubes in the style of Nicotiana alata Link et Otto was studied using specific monoclonal antibodies. The antibodies were raised against a laminarinhaemocyanin conjugate. One antibody selected for further characterization was specific for (1→3)-β-glucans and showed no binding activity against either a cellopentaose-bovine serum albumin (BSA) conjugate or a (1→3, 1→4)-β-glucan-BSA conjugate. Binding was inhibited by (1→3)-β-oligoglucosides (DP, 3–6) with maximum competition being shown by laminaripentaose and laminarihexaose, indicating that the epitope included at least five (1→3)-β-linked glucopyranose residues. The monoclonal antibody was determined to have an affinity constant for laminarihexaose of 2.7. 104M−1. When used with a second-stage gold-labelled, rabbit anti-mouse antibody, the monoclonal antibody probe specifically located the (1→3)-β-glucan in the inner wall layer of thin sections of the N. alata pollen tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194507
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    The application of Sirofluor, a chemically defined fluorochrome from aniline blue for the histochemical detection of callose (1984)
    Springer
    Protoplasma 122 (1984), S. 191-195 
    Details
    ISSN: 1615-6102
    Keywords: Aniline blue ; Callose ; Cell walls ; Fluorochrome ; (1 → 3)-β-glucan ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sirofluor, a chemically defined fluorochrome from aniline blue in aqueous unbuffered solutions, complexes with isolated (1 → 3)-β-glucans, but not (1 → 4)-β-glucans, after embedding in JB-4 resin and sectioning. Under these conditions, callose deposits in plant tissues give a brilliant yellow fluorescence with essentially no background fluorescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281696
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    Paper (German National Licenses)
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