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  • 1
    Electronic Resource
    Electronic Resource
    The cryopreservation of Chlorella (1977)
    Springer
    Archives of microbiology 114 (1977), S. 249-254 
    Details
    ISSN: 1432-072X
    Keywords: Chlorella ; Cryopreservation ; Fatty acid analysis ; Preeze-fracture electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Following a shift from autotrophic to heterotrophic nutrition, cells of Chlorella protothecoides become sensitive to the stresses of freezing and thawing. The injury then observed at slow rates of cooling cannot be explained by the cellular response to hypertonic solutions, and at faster cooling rates intracellular ice formation was not demonstrated to be damaging. These findings are at variance with suggested mechanisms of injury in other cellular systems. The results are compared with alterations in ultrastructure and in the composition of the cellular fatty acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446869
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The cryopreservation ofChlorella (1978)
    Springer
    Archives of microbiology 119 (1978), S. 153-156 
    Details
    ISSN: 1432-072X
    Keywords: Chlorella ; Cryopreservation ; Fatty acids analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Following growth under sub-optimal concentrations of nutrients, cells ofChlorella emersonii accumulated lipid and became more resistant to the damage caused by freezing and thawing. These results suggest that the factor responsible for the cold hardening of someChlorella spp is not the effect of low temperatures per se but simply that of the reduced metabolic rate. Evidence is given that the post-thaw injury observed following rapid rates of cooling is associated with the vacuole.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00964266
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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