ISSN:
0749-1581
Keywords:
NMR imaging
;
Freezing
;
Dimethyl sulphoxide
;
Cryoprotectant
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
NMR microscopic imaging is shown to be an excellent probe of the freezing and thawing phenomena of liquids in heterogeneous chemical systems, and, in addition, is capable of monitoring the freezing-thawing of mixed solvent systems. In the model mixed solvent system Me2SO-water, the chemical shift specific slice selective (C4S) imaging technique is used to obtain the spatial distributions of the concentrations and mobilities of the two individual solvents in test samples. Measurements of relaxation parameters make it possible to choose values of imaging parameters TE and TR to ensure quantitative reliability of the images. Images of test samples generated at various temperatures demonstrate the qualitative relationship between image intensity and the Me2SO-water phase diagram. This series of experiments verifies that NMR microscopic imaging will be a powerful tool for the study of liquids within solid matrices, and in particular of cryoprotectant solvents in biological systems.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260320504
Permalink
