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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 133 (1996), S. 139-142 
    ISSN: 1573-0832
    Keywords: Cryptococcus neoformans ; growth ; medium ; physiology ; thiamine ; vitamins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The use of a simple synthetic medium is essential for study on the growth and physiology ofCryptococcus neoformans. In the present study, a minimal synthetic liquid medium (MSM) was tested for the growth of 23C. neoformans strains. This medium contained a low concentration of glucose, ammonium sulphate and inorganic salts with a pH value of 4.5, but no amino acids or vitamins. The strains were starved for 4 days to eliminate nutrients which might have been carried over from their pre-culture medium. Then, they were inoculated in the MSM at an initial OD of 0.020 at 550 nm and incubated at 37 °C for 20 days. Cell growth was generally monitored daily by measuring the absorbance at 550 nm. The medium supported the growth of the strains tested and gave an average final OD of 0.500. The results obtained indicate thatC. neoformans may be autotrophic with respect to vitamins and in particular to thiamine. The MSM medium is easy to prepare and store. It is highly reproducible and useful for studies on the growth and physiology ofC. neoformans.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 136 (1996), S. 119-123 
    ISSN: 1573-0832
    Keywords: Cryptococcus neoformans ; phospolipase ; virulence ; AIDS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Phospholipases have only been detected in a few fungi and yeasts, in particular in Candida albicans. Secreted phospholipases are considered by some researchers to be a potential factor of virulence and pathogenicity in C. albicans. Twenty-three Cryptococcus neoformans strains were tested in order to observe phospholipase production. Twenty-two of the 23 strains tested were able to produce phospholipases, and the ratio diameter of the colony to total diameter of the colony plus zone of precipitation (Pz) ranged between 0.271 and 0.949. C. neoformans, just like C. albicans, can be divided on the basis of the Pz into different strains according to their virulence and pathogenicity. There also appeared to be a correlation between the phospholipase production and the size of the capsule in the strains isolated from AIDS patients. For this reason, further studies on C. neoformans phospholipase activity would be useful in evaluating the virulence of different strains.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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