Electronic Resource
Springer
Development genes and evolution
197 (1988), S. 190-192
ISSN:
1432-041X
Keywords:
Xenopus laevis
;
Free lens
;
Crystallin
;
Indirect immunofluorescence
;
Antigen
;
Antiserum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The ontogeny and localization of crystallins during free lens development (i.e. lens development without the optic vesicle) were investigated in Xenopus laevis using the indirect immunofluorescence staining method with an antiserum raised against homologous total lens soluble proteins. Since the developing free lenses pass through stages similar to those of the lenses regenerated from the inner cell layer of the outer cornea following lentectomy in the same species Freeman's classification was used to identify the stages of free lens development. The first appearance of a positive reaction occurred at early stage IV in a number of cells in an area where future lens fibre cells would develop. With further differentiation of the free lens more and more cells in the fibre area started to show a positive reaction and the first positive reaction in the epithelium was observed late in stage V. Histological examination revealed that a fully differentiated free lens and a normally developed lens are similar but that the free lens is smaller.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00427923
Permalink
| Library |
Location |
Call Number |
Volume/Issue/Year |
Availability |
