ISSN:
1573-5028
Keywords:
malate synthase
;
gene structure
;
glyoxylate cycle
;
glyoxysomes
;
Cucumis sativus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The complete sequences of a full-length cDNA clone and a genomic clone encoding the Cucumis sativus glyoxysomal enzyme malate synthase, have been determined. The sequences have enabled us to identify putative control regions at the 5′ end of the gene, three introns, and possible alternative polyadenylation sites at the 3′ end. The deduced amino acid sequence predicts a polypeptide of 64961 molecular weight, which has 48% identity with that of Escherichia coli. Comparison of the sequence of malate synthase from cucumber with that from E. coli and with other glyoxysomal and peroxisomal enzymes, shows that a conserved C-terminal tripeptide is a common feature of those enzymes imported into microbodies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00016022
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