ISSN:
1432-072X
Keywords:
Bacillus coagulans
;
Facultative thermophile
;
Respiratory chain
;
Inhibitors
;
Cytochromes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Two oxidases were found to be present in membranes from the facultative thermophile Bacillus coagulans grown at 55°C, compared to one in cells grown at 37°C. Cytochrome spectra and inhibitors of the respiratory chain identified them as cytochrome oxidases aa 3 and d. Both were present in membranes from 55°C grown cells, but only cytochrome oxidase aa 3 was found in membranes from 37°C grown cells. The presence of cytochrome d in 55°C grown cultures was found to be due to decreased oxygen tension and not to the high growth temperature. This was confirmed by (a) induction of cytochrome d at 37°C under conditions of oxygen limitation and (b) its repression at 55°C under conditions of high aeration and its subsequent induction on lowering the dissolved oxygen concentration in chemostat cultures. Two cytochromes b (λmax 558 and λmax 562) were present in both 37°C and 55°C grown cells. Results from the inhibition of substrate oxidation by membranes suggested different pathways of electron transport by the respiratory chain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00470869
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