ISSN:
1432-2242
Keywords:
Potato
;
Somatic fusion
;
Cytoplasm Starch
;
Variability
;
Field evaluation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The nuclear and cytoplasmic composition of five different fusion combinations, consisting of up to 50 hybrid regenerants each, was characterized by RFLP analysis. Simultaneously, the hybrid clones of four fusion combinations were evaluated in field experiments for yield and starch content. Predominantly complete chloroplast segregation was found with a 1∶1 ratio, in all but one fusion combination. Mitochondria, in contrast revealed up to 75% recombination, as proven by the partial addition of parental banding patterns and the altered assignment of the same genotypes with different probes. Newly occuring DNA bands were also indicative of rearrangements in the mitochondrial genome. Correlations between RFLP data and field parameters were calculated. Deviating RFLP patterns of the nuclear genome did not influence yield parameters. Also the assignment of hybrids to different chloroplast genotypes did not affect yield or starch content. However, mitochondrial types could be distinguished with respect to starch content and tuber yield. The more thorough analysis of mitochondrial composition, with different probes homologous to coding regions, revealed a relationship between the homogeneity of the mt genome and the yield level.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00224512
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